LOCUS       sequence-149304-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress TIP60 212-364 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: p3xFLAG-CMV TIP60 212-364
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(662..727)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(757..808)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(786..806)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttccacttgc cccttgctcc ataccacccc cctccacccc ataatattat agaaggacac
       61 ctagtcagac aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt
      121 ggcaacttcc agggccagga gaggcactgg ggaggggtca cagggatgcc acccgggatc
      181 ctctagatca gtcttccgtt gattctttct ccttggagaa gtagcccacg atgtggaagc
      241 ccttacagtc atactctgtc atgacgtaga agaggaaagg gtctgtgtca tagtacagtg
      301 tcttatggtc aaggaaacac ttggccaaaa gacacaggtt ctgggaataa ctcttgttct
      361 tacgtccatc aatctcaaag aaggagatgg tgcccttgcg gtaaatctca ttgcctggag
      421 gatgtcgtag gtcacacttg gtcaaatgac gctgaagaca cttgagacta cggccgtact
      481 tgaggcagaa ctcgcacagg tagaggacag gcaatgtggt gagttcctgt gggtacgggg
      541 agaagtacca cggcttgagg cggtgccggc ccagctcaat gcactcaatg ttcttcatcc
      601 gggtgacgat gtcgtcgtgg cttcgatcag acaccaggct ggcagtcatt gcggccgcaa
      661 gcttgtcatc gtcatccttg taatcgatgt catgatcttt ataatcaccg tcatggtctt
      721 tgtagtccat ggttaattct gacggttcac taaacgagct ctgcttatat agacctccca
      781 ccgtacacgc ctaccgccca tttgcgtcaa cggg
//
