LOCUS       sequence-149324-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress  TIP60 390-513 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Lee MS, Seo J, Choi DY, Lee EW, Ko A, Ha NC, Yoon JB, Lee HW, Kim 
            KP, Song J
  TITLE     Stabilization of p21 (Cip1/WAF1) following Tip60-dependent 
            acetylation is required for p21-mediated DNA damage response.
  JOURNAL   Cell Death Differ. 2013 Apr;20(4):620-9. doi: 10.1038/cdd.2012.159. 
            Epub 2012 Dec 14.
  PUBMED    23238566
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2012.159"; journalName: "Cell Death Differ"; date: 
            "2013-04"; volume: "20"; issue: "4"; pages: "620-9"
COMMENT     Sequence Label: p3xFLAG-CMV TIP60 390-513
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(576..641)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(671..771)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     promoter        complement(674..877)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(700..720)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccacttgccc cttgctccat accacccccc tccaccccat aatattatag aaggacacct
       61 agtcagacaa aatgatgcaa cttaatttta ttaggacaag gctggtgggc actggagtgg
      121 caacttccag ggccaggaga ggcactgggg aggggtcaca gggatgccac ccgggatcct
      181 ctagatcacc acttccccct cttgctccag tccttgggag tgaagtgcag acacttggag
      241 tcgatccgca ggagccgctt gagcatggcc cgctcatggc catccacgat gtcctctgac
      301 agtgtgagga tgtactggcc cttgtagtag ttgatgagat tgaggtactg cagagtggag
      361 atgacatcct ccttcttgat gctggtgatt tcactaatct cattgatggt gatctgtggc
      421 ctctccccgc tctccgactt cagccccatc aggatctcca ggatggtctg ggaccagtag
      481 cttcgatagg ataggaggcc aaggtctgag aggggcttct caggggtccc tgttttccct
      541 tccactttgg agagttcata cattgcggcc gcaagcttgt catcgtcatc cttgtaatcg
      601 atgtcatgat ctttataatc accgtcatgg tctttgtagt ccatggttaa ttctgacggt
      661 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      721 tcaacggggc ggggtttatt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      781 aaactcccat tgacgtcaat ggggtggaga ctttggaaaa tccccgtgag tcaaaccgct
      841 atccacgccc attggtgtac tgccaaaacc cgcatcacca tggtaatagc
//
