LOCUS       sequence-149345-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_Cas9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctggagcact gcctgaatca ctttttttca ggttggaccg gtatggatta caaagacgat
       61 gacgataagg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg
      121 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc
      181 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca
      241 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg
      301 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc
      361 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc
      421 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg
      481 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg
      541 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac
      601 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa
      661 aaccccatca acgccagcgg cgtggacgcc aag
//
