LOCUS       sequence-149346-         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_Cas9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcggctgatc tatctggccc tggcccacat gatcacgttc cggggccact tcctgatcga
       61 gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc tggtgcagac
      121 ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg ccaaggccat
      181 cctgtctgcc agactgagca agagcagacg gctggaaaat ctgatcgccc agctgcccgg
      241 cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc tgacccccaa
      301 cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca aggacaccta
      361 cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg acctgtttct
      421 ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag tgaacaccga
      481 gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc accaccagga
      541 cctgaccctg ctgaaagctc tcgtgc
//
