LOCUS       sequence-149354-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-PK9
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..86
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             90..131
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             132..173
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             180..221
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             225..266
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             267..308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             315..356
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             360..401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             402..443
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      473..660
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(528..552)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cgtacgctgc aggtcgacgg atccccgggt taattaacgc tagcggtaag cctattccta
       61 accctctttt gggtctcgat tctacaggtg gaaaaccaat ccccaaccca ctcctcggcc
      121 ttgactcaac tggcaaaccc ataccaaatc ctttacttgg attagatagt acctctagcg
      181 gtaagcctat tcctaaccct cttttgggtc tcgattctac aggtggaaaa ccaatcccca
      241 acccactcct cggccttgac tcaactggca aacccatacc aaatccttta cttggattag
      301 atagtacctc tagcggtaag cctattccta accctctttt gggtctcgat tctacaggtg
      361 gaaaaccaat ccccaaccca ctcctcggcc ttgactcaac tggcaaaccc ataccaaatc
      421 ctttacttgg attagatagt acctctagat gaggcgcgcc acttctaaat aagcgaattt
      481 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
      541 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
      601 ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca cctctaccgg
      661 cagatccgct aggagatctg agctcgatat caagcttagc tacaaatccc actggctata
      721 tgtatgcatt tgtgttaaaa aagtttgtat agattattta atctactcag cattctttct
      781 ctaaatagga atttgttact taatggagaa aaaaatgttt cgatttacct agtgtatttg
      841 tttgtatact cacgtttaat ttcaaacatc cattctatct tgtgtaattt ttggcatggt
      901 gaaaaagata atcagcctta taatctttac aaaagtaaga aattctgtaa ataagcctta
      961 atgcccttgc tttaaa
//
