LOCUS       sequence-149357-         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3d-PK12
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..81
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             85..126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             127..168
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             175..216
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             220..261
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             262..303
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             310..351
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             355..396
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             397..438
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             445..486
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             490..531
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             532..573
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      603..790
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(658..682)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gctgcaggtc gacggatccc cgggttaatt aacgctagcg gtaagcctat tcctaaccct
       61 cttttgggtc tcgattctac aggtggaaaa ccaatcccca acccactcct cggccttgac
      121 tcaactggca aacccatacc aaatccttta cttggattag atagtacctc tagcggtaag
      181 cctattccta accctctttt gggtctcgat tctacaggtg gaaaaccaat ccccaaccca
      241 ctcctcggcc ttgactcaac tggcaaaccc ataccaaatc ctttacttgg attagatagt
      301 acctctagcg gtaagcctat tcctaaccct cttttgggtc tcgattctac aggtggaaaa
      361 ccaatcccca acccactcct cggccttgac tcaactggca aacccatacc aaatccttta
      421 cttggattag atagtacctc tagcggtaag cctattccta accctctttt gggtctcgat
      481 tctacaggtg gaaaaccaat ccccaaccca ctcctcggcc ttgactcaac tggcaaaccc
      541 ataccaaatc ctttacttgg attagatagt acctctagat gaggcgcgcc acttctaaat
      601 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
      661 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
      721 ctttcctgta ggtcaggttg ctttctcagg tatagtatga ggtcgctctt attgaccaca
      781 cctctaccgg cagatccgct aggagatctg agctcgatat caagcttagc tacaaatccc
      841 actggctata tgtatgcatt tgtgttaaaa aagtttgtat agattattta atctactcag
      901 cattctttct ctaaatagga atttgttact taat
//
