LOCUS       sequence-149395-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express mCherry-Rab9a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: mCherry-Rab9a
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..745
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(183..201)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga
       61 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      181 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
      241 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      301 gaagctgtcc ttccccgagg gcttcaattg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      421 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagtgtcgta ccatgggctg
      481 ggaggcctcc actgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
      541 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
      601 caagaagccc gtgcagctgc ccggcgccta caacgtcgac atcaagttgg acatcctttc
      661 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
      721 cggcggcatg gacgagctgt acaagtccgg actcagatct cgagctcgca tggcaggaaa
//
