LOCUS       sequence-149423-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress ARF1-64 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Ko A, Shin JY, Seo J, Lee KD, Lee EW, Lee MS, Lee HW, Choi IJ, Jeong
            JS, Chun KH, Song J
  TITLE     Acceleration of gastric tumorigenesis through MKRN1-mediated 
            posttranslational regulation of p14ARF.
  JOURNAL   J Natl Cancer Inst. 2012 Nov 7;104(21):1660-72. doi: 
            10.1093/jnci/djs424. Epub 2012 Oct 25.
  PUBMED    23104211
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jnci/djs424"; journalName: "J Natl Cancer Inst"; date: 
            "2012-11-7- 7"; volume: "104"; issue: "21"; pages: "1660-72"
COMMENT     Sequence Label: pCS3-MT-BX/ARF1-64
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             120..149
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             159..188
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             198..227
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             237..266
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             276..305
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             339..368
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    659..793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(683..702)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     737..756
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(907..927)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(907..925)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattt aaagctatgg
      121 agcaaaagct catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag
      181 aggacttgaa tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc
      241 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
      301 acttgaatga aatggagagc ttgggcgacc tcaccatgga gcaaaagctc atttctgaag
      361 aggacttgaa tttagagatc tcgagctcaa gcttcgaatt catggtgcgc aggttcttgg
      421 tgaccctccg gattcggcgc gcgtgcggcc cgccgcgagt gagggttttc gtggttcaca
      481 tcccgcggct cacgggggag tgggcagcgc caggggcgcc cgccgctgtg gccctcgtgc
      541 tgatgctact gaggagccag cgtctagggc agcagccgct tcctagaaga ccataactcg
      601 acggtaccgc gggcccggga tccaccggat ctagaactat agtgagtcgt attacgtaga
      661 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      721 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      781 caataaacaa gttaacaaca acaattgcat tcattttatg tttcacgttc agggggaggt
      841 gtggggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt
      901 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
//
