LOCUS       sequence-149455-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS 
            and a sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1811_arC9_noNLS_human
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgataaggac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
       61 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
      121 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
      181 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
      241 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
      301 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
      361 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
      421 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
      481 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc
//
