LOCUS       sequence-149464-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS 
            and a sgRNA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, 
            Savage DF
  TITLE     Profiling of engineering hotspots identifies an allosteric 
            CRISPR-Cas9 switch.
  JOURNAL   Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
  PUBMED    27136077
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528."
COMMENT     Sequence Label: pBLO1806_Cas9_2xNLS_human
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
       61 acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca
      121 accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt
      181 ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga
      241 agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca
      301 agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg
      361 acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg
      421 ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca
      481 ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga
      541 ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg
      601 accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct
      661 acaagttcat caagcccatc ctggaaaaga tgg
//
