LOCUS       sequence-149551-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress PPARgamma1 in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Kim JH, Park KW, Lee EW, Jang WS, Seo J, Shin S, Hwang KA, Song J
  TITLE     Suppression of PPARgamma through MKRN1-mediated ubiquitination and 
            degradation prevents adipocyte differentiation.
  JOURNAL   Cell Death Differ. 2014 Apr;21(4):594-603. doi: 
            10.1038/cdd.2013.181. Epub 2013 Dec 13.
  PUBMED    24336050
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cdd.2013.181"; journalName: "Cell Death Differ"; date: 
            "2014-04"; volume: "21"; issue: "4"; pages: "594-603"
COMMENT     Sequence Label: PCS4 3XFLAG-PPARgamma1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..72
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     54..71
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             148..171
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             175..198
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             202..225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcatgctcgt ttagggaacc gccattctgc ctggggacgt cggagcaagc ttgatttagg
       61 tgacactata gaatacaagc tacttgttct ttttgcagga tcccatcgat tgtacagata
      121 tctggcgcgc ctaccatggg gcccggcgac tacaaggatg acgatgacaa gggcgactac
      181 aaggatgacg atgacaaggg cgactacaag gatgacgatg acaagggccg gcctaagatc
      241 tggaattcaa ggcctctcga gatgaccatg gttgacacag agatgccatt ctggcccacc
      301 aacttcggaa tcagctctgt ggacctctcc gtgatggaag accactcgca ttcctttgac
      361 atcaagccct ttaccacagt tgatttctcc agcatttctg ctccacacta tgaagacatt
      421 ccattcacaa gagctgaccc aatggttgct gattacaaat atgacctgaa gctccaagaa
      481 taccaaagtg cgatcaaagt agaacctgca tctccacctt attattctga aaagacccag
      541 ctctacaaca ggcctcatga agaaccttct aactccctca tggccattga gtgccgagtc
      601 tgtggggata aagcatcagg cttccactat ggagttcatg cttgtgaagg atgcaagggt
      661 tttttccgaa gaaccatccg attgaagctt atttatgata ggtgtgatct taactgccgg
      721 atccacaaaa aaagtagaaa taaatgtcag tactgtcggt ttcagaagtg ccttgctgtg
      781 gggatgtctc acaatgccat caggtttggg cggatgccac aggccgagaa ggagaagctg
      841 ttggcggaga tctccagtga tatcgaccag ctga
//
