LOCUS       sequence-149593-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3b-FL3
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             77..124
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     terminator      154..341
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(209..233)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        400..743
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 gtacgctgca ggtcgacgga tccccgggtt aattaacgct agcatgggtg actacaagga
       61 cgacgatgac aagggagatt acaaagatga cgacgataag gactacaagg acgacgatga
      121 caagtctaga tgaggcgcgc cacttctaaa taagcgaatt tcttatgatt tatgattttt
      181 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      241 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      301 gtatagtatg aggtcgctct tattgaccac acctctaccg gcagatccgc taggagatct
      361 gtttagcttg cctcgtcccc gccgggtcac ccggtcagcg acatggaggc ccagaatacc
      421 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
      481 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
      541 cgaagcaaaa attacggctc ctcgctgcgg acctgcgagc agggaaacgc tcccctcaca
      601 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
      661 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
      721 atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg acgtctgtcg
      781 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
      841 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
      901 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
      961 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     1021 cccgccgtgc acagggtgtc acgttg
//
