LOCUS       sequence-149667-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutive Cre expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNXRVd-HACre
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..124
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             161..187
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             206..226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct aggatctcga gctcaagctt cgtacgctgc
       61 aggtcgacgg atccccgggt taattaacgc tagcatgtac ccatacgatg ttcctgacta
      121 tgcgggctac ccgtatgacg tgccggacta tgcaggagcc tatccttatg acgttccaga
      181 ttacgctggt tctagatgta ctccaccaaa gaagaagaga aaggttgccc ctagcatggc
      241 caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga
      301 ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg
      361 gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa
      421 atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg
      481 tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc
      541 cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa
      601 agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt
      661 cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc
      721 atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt
      781 taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac
      841 gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga
      901 gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt
      961 cagaaaaaat ggtgttgc
//
