LOCUS       sequence-149698-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized LshC2c2 for purification in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
            DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, 
            Regev A, Lander ES, Koonin EV, Zhang F
  TITLE     C2c2 is a single-component programmable RNA-guided RNA-targeting 
            CRISPR effector.
  JOURNAL   Science. 2016 Jun 2. pii: aaf5573.
  PUBMED    27256883
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Jun 2. pii: aaf5573."
COMMENT     Sequence Label: pC001 - huLshC2C2-MBP for bacterial expression
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..96
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtgattaatg ccgcatcagg tcgtcagacc gttgatgaag ccctgaaaga tgcccagacc
       61 ggtgcaagca ccggatccct ggttccgcgt ggtagcgcta gcgaaaatct gtattttcag
      121 ggaatgggga acctgttcgg acacaagcgg tggtacgagg tgcgagataa gaaagacttc
      181 aagatcaagc gaaaggtgaa agtcaagcgg aattatgacg ggaacaagta cattctgaac
      241 atcaacgaaa acaacaacaa agagaagatc gacaacaaca agttcatcag aaagtacatc
      301 aactacaaga aaaacgataa tattctgaag gagttcacta ggaaatttca tgcaggaaat
      361 atcctgttca aactgaaggg caaagaaggg atcattagaa ttgagaataa cgacgacttc
      421 ctggagacag aggaagtggt gctgtatatc gaggcctacg gcaagagcga gaagctgaaa
      481 gcactgggga tcacaaagaa aaagatcatt gacgaggcca tcaggcaggg cattactaag
      541 gacgataaaa agatcgagat caagcgacag gagaacgagg aagagatcga aattgacatc
      601 cgggatgagt acactaataa gaccctgaac gactgctcca tcattctgcg catcattgaa
      661 aacgatgaac tggagacaaa gaagagcatc tacgagatct tcaagaacat caatatgagc
      721 ctgtataaga tcatcgagaa gatcatcgaa aacgagacag aaaaggtgtt tgaaaataga
      781 tactacgaag agcacctgag ggagaagctg ctgaaagacg ataagattga cgtgatcctg
      841 accaacttca tggaaatccg ggagaagatc aagtctaatc tggagatcct gggcttcgtg
      901 aagttttacc tgaacgtcgg cggggacaaa aagaaaagta aaaataagaa aatg
//
