LOCUS       sequence-149794-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, 
            Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array
            driven by its native leader, and the tmRNA::td intron construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Silas S, Mohr G, Sidote DJ, Markham LM, Sanchez-Amat A, Bhaya D, 
            Lambowitz AM, Fire AZ
  TITLE     Direct CRISPR spacer acquisition from RNA by a natural reverse 
            transcriptase-Cas1 fusion protein.
  JOURNAL   Science. 2016 Feb 26;351(6276):aad4234. doi: 
            10.1126/science.aad4234.
  PUBMED    26917774
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-02-26"; volume: "351(6276):aad4234. doi"; pages: " 
            10.1126/science.aad4234"
COMMENT     Sequence Label: pV92.2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..32
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc tcctttactc
       61 atggttaatt cctcctgtca taacaggacg gcggcgggga acaccgcaat ggggtttccc
      121 caaacgtctt ggctgtgttt tctggattct ttgtttaacc aatagaagcg caggctatct
      181 gcttcttctg tgtatttgct gcatttttta cgcagcttat gcagctcgtt ttcatctttt
      241 aggctgattt cgaataccga gtactggact cgatcgccat attctaagag taaatttgag
      301 agcttacggc gttttttatc gtcttcgatg tcgaaacagg caaggtatat tctcatcttt
      361 tagggctcca agcgataaag cgttgttgca ttttgcagct tagggctaaa cggtaagatt
      421 ggttgctcat ttcggtgagc agactgtttc ctcccttagg cactttagtc agttgaacca
      481 agagttcttt tagaagtgtt tttctcgcgc cactgctcat ttcgcaacca ttttctttga
      541 tgacaaagtc atccttatgt atttggtgac gatttattag ggttaagacg acgtgttcga
      601 caaggtaacg ccaaggttcc attaggtcag aagctagggt gtgatggtaa ccatgacgct
      661 ggtgcagtgc gccttgccaa gggtagagtc caaccgttct attgacactg tctactaagc
      721 tgtatagata ggtgtagcct aatgaaagta gggcgttgat cgggtctttg ggagggcgtc
      781 tattacgccc tgtaaagccc caatcttctt cgagtatacg ctggaagaat gcccacagtt
      841 gtttgctggc ttggccttca tagcctcgta attgatctaa tgtttctgcc cgtaagcgtt
      901 tttcgtccaa gcgttgaatt ttgttcagct caggggcatc ttttt
//
