LOCUS       sequence-149811-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To overexpress RIPK3 2KR(55,363) in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Seo J, Lee EW, Sung H, Seong D, Dondelinger Y, Shin J, Jeong M, Lee 
            HK, Kim JH, Han SY, Lee C, Seong JK, Vandenabeele P, Song J
  TITLE     CHIP controls necroptosis through ubiquitylation- and 
            lysosome-dependent degradation of RIPK3.
  JOURNAL   Nat Cell Biol. 2016 Mar;18(3):291-302. doi: 10.1038/ncb3314. Epub 
            2016 Feb 22.
  PUBMED    26900751
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3314"; 
            journalName: "Nat Cell Biol"; date: "2016-03"; volume: "18"; issue: 
            "3"; pages: "291-302"
COMMENT     Sequence Label: pcDNA3-FLAG-RIPK3 2KR(55,363)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      87..96
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
       61 tcactatagg gagacccaag cttgccgcca ccatggatta caaggatgac gacgataaga
      121 gcgcgggcgg atccgaattc tgcagatatc catcacactg gcggccgctc gagatgtcgt
      181 gcgtcaagtt atggcccagc ggtgcccccg cccccttggt gtccatcgag gaactggaga
      241 accaggagct cgtcggcaaa ggcgggttcg gcacagtgtt ccgggcgcaa cataggaagt
      301 ggggctacga tgtggcggtc aagatcgtaa actcgcgggc gatatccagg gaggtcaagg
      361 ccatggcaag tctggataac gaattcgtgc tgcgcctaga aggggttatc gagaaggtga
      421 actgggacca agatcccaag ccggctctgg tgactaaatt catggagaac ggctccttgt
      481 cggggctgct gcagtcccag tgccctcggc cctggccgct cctttgccgc ctgctgaaag
      541 aagtggtgct tgggatgttt tacctgcacg accagaaccc ggtgctcctg caccgggacc
      601 tcaagccatc caacgtcctg ctggacccag agctgcacgt caagctggca gattttggcc
      661 tgtccacatt tcagggaggc tcacagtcag ggacagggtc cggggagcca gggggcaccc
      721 tgggctactt ggccccagaa ctgtttgtta acgtaaaccg gaaggcctcc acagccagtg
      781 acgtctacag ctttcgggat cctaatgtgg gcagtgcttg ctggaaaaga
//
