LOCUS       sequence-150051-        8966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and mCherry protein; flanked by Tol2 
            sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-ubb-cytoBirA-2a-mCherry
FEATURES             Location/Qualifiers
     source          1..8966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      353..941
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     842..861
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1095..1112
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1229..1250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1265..1270,1271..1288,1289..1295)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1308..1330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1327..1343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1327..1343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1362..1382
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1364..1382
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1419..1435
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1419..1435
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(1469..1480,1481..1668)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     regulatory      5172..5181
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5191..5217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5224..5250
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5257..5283
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5299..6261
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5299..6261
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             6277..6330
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6337..7047
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6750..6769
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    7099..7220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7110..7129)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7164..7183
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7337..7474,7475..7486)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7544..7561)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7544..7560)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7553..7575)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7702..8157
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7789..7808)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7999..8020
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8183..8287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8288..8356,8357..8966,1..182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8506..8525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
       61 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
      121 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
      181 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
      241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
      301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
      541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     1141 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1201 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     1261 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     1321 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     1381 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatctgctg
     1441 ggcttgctga aggtaggggg tcaagaacca gaggtgtaaa gtacttgagt aattttactt
     1501 gattactgta cttaagtatt atttttgggg atttttactt tacttgagta caattaaaaa
     1561 tcaatacttt tacttttact taattacatt tttttagaaa aaaaagtact ttttactcct
     1621 tacaatttta tttacagtca aaaagtactt attttttgga gatcacttgg gcccggctcg
     1681 agaccagcaa agttctagaa tttgtcgaaa catttatgtt atatatttcc tgaaaaaaat
     1741 tctgagtaag ttcttaagtg ttattgccag caacataaac aacagacggc aaaatgaata
     1801 aatgataaca aagcagtagg cttaaataaa cctaattttt ataggctgtt ctctacaacc
     1861 ctcaaacagt gattagtttt gtacttataa acttgccctt tcattcatat ttcaagaaaa
     1921 ttggttcaga agatctggat attctagcag ttgttcaagc tcatggaggg atcagtgacc
     1981 tgattccaca atgactaggc ctaatccaga aattagatga ctgtcaacat aaaaaggcac
     2041 agcactcact agctgcccta tatattttat tatattttac atatattatt ttatttattt
     2101 agctctgagt gctgtacttt ctggttaaag aaaactgctt acaacagcta acctgtacta
     2161 cctcaggctc agggaatttg gaacaggttt gtctggtttg tttctttaac catgcatgct
     2221 tgttttcaac tatggcaaca cagtcacatg ggacattaca gaaatgattt gtcgatgaca
     2281 tgcgactttt ctttaataaa gcgcaaagat cccaaaaagc aaacttttaa caaaaatcat
     2341 ataattatat tttcaatcca gctttgtagc aactttgtgc tgctgttcac tcagcaacag
     2401 atagtcagta taaggtcagt gtgtctcaaa gcagtgccat ctgtttcaca cattgcgttc
     2461 tatatataag tgtgctggtt gacacgacac tgtataaggc ctaggctaaa acacaaacaa
     2521 tgtagaatga cactgtgttt tttttgtaaa caaatgttgt ttttggttaa acatctttgt
     2581 gaaaacatcc tcctgtcatg tatttgctat attcaaatgt taaacccgtg cagaatagaa
     2641 catatacaaa aaaaaacaac acaacacatt tttaaacatt attaaatatc aagtattgct
     2701 ggcagttctg tttctgtttt acagtaccct ttgccacagt tctccgcttt tcctggtcca
     2761 gattccacaa gtctgattca ccaatagcaa agcgaataaa caaccaaagc agccaatcac
     2821 tgcttgtaga ctgtcctgcg agaccggccc attccagcac attctggaaa cttcctttat
     2881 atgataatta taaatacatt taaattattg atacaaaaca tgtaattcct agaacataac
     2941 catagcaatc attagttttc agggtaatta tgtattttta ggatttgact gcggaaagat
     3001 ctggtcatgt gacgtctcat gaacgtcacg gccctgggtt tctataaata cagtaggact
     3061 ctcgaccatc ggcagatttt tcgaagaaga agatcagttt caggagccgt actgttccgt
     3121 ttcaacgcaa atattaacgg taagagcgaa tttcctagtt tgttttcatg ccattcttta
     3181 aaaccatagc gtattacttt aattatagta aactttcgct ttctttatta caagagacgt
     3241 tttgtgttga ttctccgcgg acattttcgg tcagacaatc agaaaatgac cgcggaggac
     3301 cagtaacttg cattacacgt aagttaaatc ttcgtgtatt aaaatggtta ggttgttaac
     3361 gtcaaatagg ttaccgtgtt tgcgtgtgat caggttggtt ttgttagatt tttgtcagta
     3421 tttttaattt atttgtttta gtttatttat tttttttgct gaatcatagt ttgtgaacaa
     3481 agaacccgga tgttacatac agtacagccg ccatgttaca gagagttata acttaatcat
     3541 tttaaaaata attttgcctt acttttagtt tgtcatgttg agaaatgagg aaatgttaaa
     3601 atgaggaaat atccaattaa tttaatatat caaaataatc catgattaca atgcactgaa
     3661 ctggagaaaa ttaagatgtt ttctagtgtc atgaaacaaa tgtaagagat gtacattgta
     3721 gatgttttat gtcaagaatt ggctagttga tgcagcatac tggcgatact cagttgtaat
     3781 aacagtaacg ttacatgtta atagactact gagtatgctg ttctgtctat gtatgctctg
     3841 taagctacga gaaggacttt tttaaacagt aaagggtgca atatttttac aaattgaatt
     3901 aaataaaggc tgtctattaa gtaatatgct tgatattttt cttacttgat cggaaataag
     3961 aaaaaatata aacgttgttg ctctaaaaat cctagttcag tttagccaac cacaaatacc
     4021 tttttgttcc tccaacagtt tttttttctt ctctataata tttggcagtc tatagtactc
     4081 caaatgtttc cccacagtct aactaattgg tacagccaaa atcatgacac ttattgcaat
     4141 aataattttg gttcattggc attgttgata gcctgtgcca ctaatatggt cgattgatca
     4201 tgcttcagga agaaaactat attgtttgat gtaagattat taaatcttca cctgcttcca
     4261 ttacaaacta ttcccatctt attgaattct ggtatgtctt aaaggattag ttcacttccc
     4321 aaatcaaaat ttacttagcc ttttttcatc catgatccct ttttttcatc attaatgaag
     4381 aaattgtttt tgaaaaagtt tcaagatttt tttctctata ttgtggagct tgttagttta
     4441 aaattccaaa atgcaatatg tggcttcaaa tggttctaaa tgatcccagt caaggaataa
     4501 cagtcttatc taatgaaacc attagacctt taaaaataaa aaaaataaaa gtatttattt
     4561 ttaaatgact gagtgattaa gttgaatttc agcgtttcct tactgtgtag aagtccttcc
     4621 ttactggccc caccctttgg ttctctgcca atctgctacc taatgtaatg ttgtggaaca
     4681 ttattattct ttatttctta attttttatt ttttatttta aaacaatgta aactgcacag
     4741 atgtgcagtt tgtttaaaat ggccaatgct ttggaaatgc atgacataat tagatttcat
     4801 gatgcacaaa gccaaatctc agagcttgtg caaaatgagc tatcatttca ctaggtaaga
     4861 ccctaaattt tcatatagga tcatttggac aattttgctg caggtaaaat gcattctata
     4921 gtccactgtc agccattgtt ttggatagta tttatttttc tctacaagta tagtcaatag
     4981 ttttctatta ttttaaaggt ttgtaacatt taagggtgac caaatgcaaa gtaaaatttc
     5041 attttcgggt gaactatctc gtttaacatg ggagaagtgc aaaacataca ttattggcta
     5101 gaacattgta gtatttttta aatggaaatg tgtgattgct aatcttactt tgaatttgtt
     5161 tacagggatc cgccaccatg gatggccacc tatgacgtcc cagattacgc aagtttgccc
     5221 ggatatgatg ttcctgatta tgctagcctc ccgggttacg atgtgcccga ctatgcctca
     5281 cttccaggca gatctcctat gaaggataac accgtgccac tgaaattgat tgccctgtta
     5341 gcgaacggtg aatttcactc tggcgagcag ttgggtgaaa cgctgggaat gagccgggcg
     5401 gctattaata aacacattca gacactgcgt gactggggcg ttgatgtctt taccgttccg
     5461 ggaaaaggat acagcctgcc tgagcctatc cagttactta atgctaaaca gatattgggt
     5521 cagctggatg gcggtagtgt agccgtgctg ccagtgattg actccacgaa tcagtacctt
     5581 cttgatcgta tcggagagct taaatcgggc gatgcttgca ttgcagaata ccagcaggct
     5641 ggccgtggtc gccggggtcg gaaatggttt tcgccttttg gcgcaaactt atatttgtcg
     5701 atgttctggc gtctggaaca aggcccggcg gcggcgattg gtttaagtct ggttatcggt
     5761 atcgtgatgg cggaagtatt acgcaagctg ggtgcagata aagttcgtgt taaatggcct
     5821 aatgacctct atctgcagga tcgcaagctg gcaggcattc tggtggagct gactggcaaa
     5881 actggcgatg cggcgcaaat agtcattgga gccgggatca acatggcaat gcgccgtgtt
     5941 gaagagagtg tcgttaatca ggggtggatc acgctgcagg aagcggggat caatctcgat
     6001 cgtaatacgt tggcggccat gctaatacgt gaattacgtg ctgcgttgga actcttcgaa
     6061 caagaaggat tggcacctta tctgtcgcgc tgggaaaagc tggataattt tattaatcgc
     6121 ccagtgaaac ttatcattgg tgataaagaa atatttggca tttcacgcgg aatagacaaa
     6181 cagggggctt tattacttga gcaggatgga ataataaaac cctggatggg cggtgaaata
     6241 tccctgcgta gtgcagaaaa agatatcgga tcaggagagg gcaggggaag tcttctaaca
     6301 tgcggggacg tggaggaaaa tcccgggccc ggaagtatgg tgtcgaaggg cgaggaggat
     6361 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     6421 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6481 gccaagctta aagtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     6541 caattcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     6601 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     6661 gtggtgaccg tgacccagga ctcctccctc caagatggcg agttcattta taaagtgaag
     6721 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     6781 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     6841 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     6901 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     6961 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     7021 ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagagtcgg ggcggccggc
     7081 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7141 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7201 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt cgggttcagg
     7261 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt acccgggttt
     7321 aaacgcatgc agatctaata ctcaagtaca attttaatgg agtacttttt tacttttact
     7381 caagtaagat tctagccaga tacttttact tttaattgag taaaattttc cctaagtact
     7441 tgtactttca cttgagtaaa atttttgagt actttttaca cctctgtcaa gaaccatatg
     7501 ccggtaccca atcgccctat aggacgtcgt attacgcgcg ctcactggcc gtcgttttac
     7561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7681 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7741 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7801 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7861 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7921 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7981 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     8041 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8101 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     8161 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8221 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8281 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8341 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8401 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8461 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8521 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8581 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8641 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8701 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8761 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8821 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8881 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8941 tctgcgctcg gcccttccgg ctggct
//
