LOCUS       sequence-150096-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5CZG (Tb427.10.7420)
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(392..412)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(425..442)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             452..474
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    489..513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(513..532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(514..532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(616..635)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatcctc gagtgcggcc gcaagcttcg tcatcagtcc acaacgagtc
       61 ccgactgccg tgcaaggtca aggtaagttt tacgaaatga cattgcctcc tgataccacg
      121 tgctgccctt cgcattgtac tccaaagcgt tcgtaatcat gcgagcgacg tcgttctgta
      181 cgtccacgtc agttgcataa gtgccctttt caatcccatc gcgtatggaa gagaggtcaa
      241 caggacgctt tatcacagtg tggtagtccg gcagttcttc ggcactaact gggtgatgaa
      301 acattccaag cttgtcaaga tcccacagac gggatacaaa aacaagacag ccattcttgt
      361 taaaagaagt atctcgctcg ttcttgctca tgccctggaa atacaagttt tctctgccgc
      421 tgctgtgatg atgatgatga tgcatggtat atctccttct taaagttaaa caaaattatt
      481 tctagagggg aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg
      541 ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
      601 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
      661 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
      721 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
      781 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
      841 gacaccatcg aa
//
