LOCUS       sequence-150120-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 5H9M (SIAH2)
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..54
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             64..81
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             82..375
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             379..399
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 actttaagaa ggagatatac catgaaaaaa catcaccatc atcatcatca tcacagcagc
       61 ggcctggttc cgcgtggtag tatgtcggac tcagaagtca atcaagaagc taagccagag
      121 gtcaagccag aagtcaagcc tgagactcac atcaatttaa aggtgtccga tggatcttca
      181 gagatcttct tcaagatcaa aaagaccact cctttaagaa ggctgatgga agcgttcgct
      241 aaaagacagg gtaaggaaat ggactcctta agattcttgt acgacggtat tagaatccaa
      301 gctgatcaga cccctgaaga tttggacatg gaggataacg atattattga ggctcacaga
      361 gaacagattg gtggaacaga aaacttgtat ttccagggcg cctcggcagt cctgtttccc
      421 tgtaagtatg ccaccacggg ctgttccctg accctgcacc atacggagaa accagaacat
      481 gaagacatat gtgaataccg tccctactcc tgcccatgtc ctggtgcttc ctgcaagtgg
      541 caggggtccc tggaagctgt gatgtcccat ctcatgcacg cccacaagag cattaccacc
      601 cttcagggag aagacatcgt ctttctagct acagacatta acttgccagg ggctgtcgac
      661 tgggtgatga tgcagtcatg ttttggccat cacttcatgc tggtgctgga gaaacaagag
      721 aagtacgaag gccaccagca gttttttgcc atcgtcctgc tcattggcac ccgcaagcaa
      781 gccgagaact ttgcctacag actggagttg aatgggaacc ggcggagatt gacctggg
//
