LOCUS       sequence-150151-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Lai JY, Poon YS, Kaiser JT, Rees DC
  TITLE     Open and shut: crystal structures of the dodecylmaltoside 
            solubilized mechanosensitive channel of small conductance from 
            Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A 
            resolutions.
  JOURNAL   Protein Sci. 2013 Apr;22(4):502-9. doi: 10.1002/pro.2222. Epub 2013 
            Feb 21.
  PUBMED    23339071
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2222"; 
            journalName: "Protein Sci"; date: "2013-04"; volume: "22"; issue: 
            "4"; pages: "502-9"
COMMENT     Sequence Label: HpMscS
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             512..529
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
      481 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atacgttaat
      541 taacatggat gaaattaaaa cgctgttagt ggattttttc ccgcaggcaa agcattttgg
      601 gataatcttg atcaaggctg tcattgtttt ttgtataggt ttttattttt cgtttttctt
      661 acggaacaaa accatgaagc ttttatccaa aaaggatgag attttagcga attttgtcgc
      721 acaggttact tttatcttaa tccttatcat caccacaatc atcgcgctca gcacgctagg
      781 cgtccaaacc acctctatta tcactgtttt aggaacggtg gggatcgctg tggcgttggc
      841 tttaaaagat tatctttcaa gcattgcggg agggataatc cttattattt tacacccttt
      901 caaaaaagga gacatcattg aaatctctgg cctagagggc aaagtagaag cacttaattt
      961 tt
//
