LOCUS       sequence-150152-        1062 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Lai JY, Poon YS, Kaiser JT, Rees DC
  TITLE     Open and shut: crystal structures of the dodecylmaltoside 
            solubilized mechanosensitive channel of small conductance from 
            Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A 
            resolutions.
  JOURNAL   Protein Sci. 2013 Apr;22(4):502-9. doi: 10.1002/pro.2222. Epub 2013 
            Feb 21.
  PUBMED    23339071
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2222"; 
            journalName: "Protein Sci"; date: "2013-04"; volume: "22"; issue: 
            "4"; pages: "502-9"
COMMENT     Sequence Label: HpMscS
FEATURES             Location/Qualifiers
     source          1..1062
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(101..148)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     144..162
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(215..232)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(281..313)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(318..341)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
      121 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
      181 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
      241 cggccgcaag cttgtcgacg gagctcgaat tcggatcccg acccatttgc tgtccaccag
      301 tcatgctagc catattactt atcgtcgtca tccttataat cgttaattaa tttaggagag
      361 tcttgatttt taatagcaat atctaactta ttgaaaggga tttcaatgtg gttagcgtct
      421 agggcgtttt tgatgcgttc aatgagttcg ctcctcacat taaagatccc gtcttcaatc
      481 tttgcccaaa ccctaatggt gaaattcaat gagctggatc caaaatccgt gattccaata
      541 aaagtgggca tgtttttatc aattttttcc attgtatcaa taacatcttt tatagtctta
      601 tgcaccagtt caatatcgct cccataccct accccacaaa cccattcaat gcgccgacac
      661 gccgtattat tgctattaat aatattagaa ttagcgacac ttctattggg taaaaccgcc
      721 aagcgtccgt catgcaagcg taaagaagtg ttaaaaaaat taagtgcttc tactttgccc
      781 tctaggccag agatttcaat gatgtctcct tttttgaaag ggtgtaaaat aataaggatt
      841 atccctcccg caatgcttga aagataatct tttaaagcca acgccacagc gatccccacc
      901 gttcctaaaa cagtgataat agaggtggtt tggacgccta gcgtgctgag cgcgatgatt
      961 gtggtgatga taaggattaa gataaaagta acctgtgcga caaaattcgc taaaatctca
     1021 tcctttttgg ataaaagctt catggttttg ttccgtaaga aa
//
