LOCUS       sequence-150166-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid used for T7 promoter driven expression 
            of recombinant proteins with N-terminal 8X His, thrombin cleavage 
            site, SUMO, and TEV cleavage site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  TITLE     SGC Toronto Q3 Q4 2015, Q1 2016
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET28-MKH8SUMO
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tttcgggctt tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc
       61 gcaagcttcg tcatcatgtg catgatctcc tcgaaaagtg ccacctgacg tcaatgccaa
      121 taggatatcg gcattttctt ttgcgttttt atttgttaac tgttaattgt ccttgttcaa
      181 ggatgctgtc tttgacaaca gatgttttct tgcctttgat gttcagcagg aagctaggcg
      241 caaacgttga ttgtttgtct gcgtagaatc ctctgtttgt catatagctt gtaatcacga
      301 cattgtttcc tttcgcttga ggtacagcga agtgtgagta agtaaaggtt acatcgttag
      361 gatcaagatc catttttaac acaaggccag ttttgttcag cggcttgtat gggccagtta
      421 aagaattaga aacataacca agcatgtaaa tatcgttaga cgtaatgccg tcaatcgtca
      481 tttttgatcc gcgggagtca gtgaacaggt accatttgcc gttcatttta aagacgttcg
      541 cgcgttcaat ttcatctgtt actgtgttag atgcaatcag cggtttcatc acttttttca
      601 gtgtgtaatc atcgtttagc tcaatcatac cgagagcgcc gtttgctaac tcagccgtgc
      661 gttttttatc gctttgcaga agtttttgac tttcttgacg gaagaatgat gtgcttttgc
      721 catagtatgc tttgttaaat aaagattctt cgccttggta gccatcttca gttccagtgt
      781 ttgcttcaaa tactaagtat ttgtggcctt t
//
