LOCUS       sequence-150172-         369 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent bicistronic vector expressing mRuby2 and GCaMP6s from 
            a single open reading frame..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Rose T, Jaepel J, Hubener M, Bonhoeffer T
  TITLE     Cell-specific restoration of stimulus preference after monocular 
            deprivation in the visual cortex.
  JOURNAL   Science. 2016 Jun 10;352(6291):1319-22. doi: 
            10.1126/science.aad3358.
  PUBMED    27284193
REFERENCE   2  (bases 1 to 369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-06-10- 10"; volume: "352"; 
            issue: "6291"; pages: "1319-22"
COMMENT     Sequence Label: pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..369
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(78..111)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgtataggat actttatacg aagttatgca gaatggtagc tggattgtag ctgctattag
       61 caatatgaaa cctcttaata acttcgtata gcatacatta tacgaagtta tactagtgcg
      121 gccgcttact tcgctgtcat catttgtaca aactcttcgt agtttacctg accatcccca
      181 tcgatgtctg cttccctgat catttcatca acctcttcat ctgttaactt ctctccaagg
      241 tttgtcatca cgtggcgaag ctctgctgca ctgatgtagc cattgccatc cttatcaaac
      301 acaccgaacg cttctctaat ttcttcttcc gtgtccctgt atttcatttt tcttgccatc
      361 attgtcagg
//
