LOCUS       sequence-150279-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 
            (G1 phase cell cycle reporter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Shcherbakova DM, Baloban M, Emelyanov AV, Brenowitz M, Guo P, 
            Verkhusha VV
  TITLE     Bright monomeric near-infrared fluorescent proteins as tags and 
            biosensors for multiscale imaging.
  JOURNAL   Nat Commun. 2016 Aug 19;7:12405. doi: 10.1038/ncomms12405.
  PUBMED    27539380
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-08-19"; volume: "7:12405. doi"; pages: " 
            10.1038/ncomms12405"
COMMENT     Sequence Label: pCSII-EF-miRFP709-hCdt(1/100)
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1681..1914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2099..2143
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2590..3752
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2821..3743
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3700..3720
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3777..4721
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 709 nm (Shcherbakova et al., 2016)"
                     /label=miRFP709
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSFIPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPIHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    5147..5735
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5200..5220)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5618..5629)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6254..6434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6460..6477)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6466..6690
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6736..7164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6823..6842)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7033..7054
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7173..7193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7178..7508
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7359..7494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7421..7440
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7556..7603
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7622..7996
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8126..8247
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8163..8182)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8217..8236
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8296..8312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8296..8312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8309..8331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8320..8336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8344..8350,8351..8368,8369..8374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8389..8410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8527..8544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8698..9286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8778..8797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9457..10248,10249..10317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10080..10099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10318..10422)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaaa ttcactttga attaattcaa
     2581 gcttcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2641 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2701 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2761 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2821 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2881 ccttgaatta cttccacctg gctccagtac gtgattcttg atcccgagct ggagccaggg
     2941 gcgggccttg cgctttagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3001 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3061 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3121 gtcttgtaaa tgcgggccag gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3181 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3241 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3301 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3361 gagcggaaag atggccgctt cccggccctg ctccaggggg ctcaaaatgg aggacgcggc
     3421 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aggggccttt ccgtcctcag
     3481 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3541 ggagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3601 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3661 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3721 aaagtttttt tcttccattt caggtgtcgt gaacacgcta ccggtctcga gaattcatgg
     3781 tagcaggtca tgcctctggc agccccgcat tcgggaccgc ctctcattcg aattgcgaac
     3841 atgaagagat ccacctcgcc ggctcgatcc agccgcatgg cgcgcttctg gtcgtcagcg
     3901 aacatgatca tcgcgtcatc caggccagcg ccaacgccgc ggaatttctg aatctcggaa
     3961 gcgtactcgg cgttccgctc gccgagatcg acggcgatct gttgatcaag atcctgccgc
     4021 atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg ctgccggatc ggcaatccct
     4081 ctacggagta ctgcggtctg atgcatcggc ctccggaagg cgggctgatc atcgaactcg
     4141 aacgtgccgg cccgtcgatc gatctgtcag gcacgctggc gccggcgctg gagcggatcc
     4201 gcacggcggg ttcactgcgc gcgctgtgcg atgacaccgt gctgctgttt cagcagtgca
     4261 ccggctacga ccgggtgatg gtgtatcgtt tcgatgagca aggccacggc ctggtattct
     4321 ccgagtgcca tgtgcctggg ctcgaatcct atttcggcaa ccgctatccg tcgtcgttca
     4381 tcccgcagat ggcgcggcag ctgtacgtgc ggcagcgcgt ccgcgtgctg gtcgacgtca
     4441 cctatcagcc ggtgccgctg gagccgcggc tgtcgccgct gaccgggcgc gatctcgaca
     4501 tgtcgggctg cttcctgcgc tcgatgtcgc cgatccatct gcagttcctg aaggacatgg
     4561 gcgtgcgcgc caccctggcg gtgtcgctgg tggtcggcgg caagctgtgg ggcctggttg
     4621 tctgtcacca ttatctgccg cgcttcatcc gtttcgagct gcgggcgatc tgcaaacggc
     4681 tcgccgaaag gatcgcgacg cggatcaccg cgcttgagag cggaggtggt ggatcaggtg
     4741 gaggaggttc tggaggtggt ggatcaggag gaggtggttc tggaggtggt ggatcgcggc
     4801 cgctcgagat ggagcagcgc cgcgtcaccg acttcttcgc gcgccgccgc cccgggcccc
     4861 cccgcatcgc gccgcccaag ctggcctgcc gcacccccag ccccgccagg cccgcactcc
     4921 gcgccccggc ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc gccgcccccg
     4981 gacgcgacca ggccaggcca ccggcccgca ggagactgcg gctgtcggtg gacgaggttt
     5041 ccagccccag tacccccgag gccccagaca tcccagcctg cccttctccg ggccagaaga
     5101 taaagaaata atctagagtt aacatcgagg gatcaagctt atcgataatc aacctctgga
     5161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5521 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5701 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgag acctggaaaa
     5761 acatggagca atcacaagta gcaacacagc agctaccaat gctgcttgtg cctggctaga
     5821 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     5881 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5941 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     6001 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     6061 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     6121 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     6181 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     6241 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6301 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6361 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6421 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6481 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6541 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6601 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6661 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6721 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6781 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6841 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt
     6901 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6961 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7021 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7081 attcttttga tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga
     7141 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7201 gtccccaggc tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7261 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7321 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7381 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7441 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7501 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     7561 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     7621 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     7681 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     7741 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     7801 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     7861 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     7921 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     7981 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     8041 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     8101 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     8161 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8221 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8281 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8341 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8401 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8461 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8521 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8581 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8641 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8701 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8761 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8821 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8881 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8941 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9001 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9061 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9121 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9181 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9241 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9301 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9361 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9421 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9481 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9541 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9601 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9661 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9721 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9781 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9841 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9901 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9961 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10021 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10081 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10141 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10201 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10261 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10321 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10381 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10441 gccacctgac gtc
//
