LOCUS       sequence-150300-        1131 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express and target GFP into the Golgi apparatus using 
            lentiviruses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1131)
  TITLE     pLV-mGFP Golgi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Golgi eGFP
FEATURES             Location/Qualifiers
     source          1..1131
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(382..403)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(643..662)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     gap             1077..1087
                     /estimated_length=11
ORIGIN
        1 agcaganctc gtttagtgac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag acaccgcccg ggcgtacggg atcccaccgg tgctcgagac tagtatgagg
      121 cttcgggagc cgctcctgag cggcagcgcc gcgatgccag gcgcgtccct acagcgggcc
      181 tgccgcctgc tcgtggccgt ctgcgctctg caccttggcg tcaccctcgt ttactacctg
      241 gctggccgcg acctgagccg cctgccccaa ctggtcggag tctccacacc gctgcagtcg
      301 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
      361 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      421 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      481 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      541 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      601 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      661 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      721 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      781 cacaacgtct atatcatggn cngacaagca gaanaacggc atcaaggtga acttcaagat
      841 ccgccacaac atcgaggacg gcagcgtgca gctcgncgac cactaccagc agaanacccc
      901 catcggcgan ggcccgngct gctgcccgan aacnctncng ancanccngt nnnctgnann
      961 aannacccnn gannnnnnga tnnncntggt cnnntngnnn tcnngaccnn nnnnnggntn
     1021 ncnctcnnnn tggncnnnnn nnnnagtann nnnnacngcn ggncnannnn nnngntnnnn
     1081 nnnnnnnann nnnatcancn nnggannnan nnnnnnnaan annncngnnn t
//
