LOCUS       sequence-150335-        9112 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a sumo-mutant form GFP-BLM with K317R and K331R 
            sumo-site mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Eladad S, Ye TZ, Hu P, Leversha M, Beresten S, Matunis MJ, Ellis NA
  TITLE     Intra-nuclear trafficking of the BLM helicase to DNA damage-induced 
            foci is regulated by SUMO modification.
  JOURNAL   Hum Mol Genet. 2005 May 15;14(10):1351-65. Epub 2005 Apr 13.
  PUBMED    15829507
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet."; date: "2005-05-15"; volume: "14(10)"; pages: "1351-65. Epub
            2005 Apr 13"
COMMENT     Sequence Label: GFP-BLM DM
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1375..1386
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5900..6021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5937..5956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5991..6010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6028..6483)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6165..6186)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6377..6396
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6510..6614
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6616..6973
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6616..6807
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6639..6659)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6824..6959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7008..7802
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7008..7802
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7062..7081)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7672..7691
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7990..8009)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8034..8081
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8410..8998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8899..8918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgagatcgag
     1381 ggtagaatgg ctgctgttcc tcaaaataat ctacaggagc aactagaacg tcactcagcc
     1441 agaacactta ataataaatt aagtctttca aaaccaaaat tttcaggttt cacttttaaa
     1501 aagaaaacat cttcagataa caatgtatct gtaactaatg tgtcagtagc aaaaacacct
     1561 gtattaagaa ataaagatgt taatgttacc gaagactttt ccttcagtga acctctaccc
     1621 aacaccacaa atcagcaaag ggtcaaggac ttctttaaaa atgctccagc aggacaggaa
     1681 acacagagag gtggatcaaa atcattattg ccagatttct tgcagactcc gaaggaagtt
     1741 gtatgcacta cccaaaacac accaactgta aagaaatccc gggatactgc tctcaagaaa
     1801 ttagaattta gttcttcacc agattcttta agtaccatca atgattggga tgatatggat
     1861 gactttgata cttctgagac ttcaaaatca tttgttacac caccccaaag tcactttgta
     1921 agagtaagca ctgctcagaa atcaaaaaag ggtaagagaa acttttttaa agcacagctt
     1981 tatacaacaa acacagtaaa gactgatttg cctccaccct cctctgaaag cgagcaaata
     2041 gatttgactg aggaacagaa ggatgactca gaatggttaa gcagcgatgt gatttgcatc
     2101 gatgatggcc ccattgctga agtgcatata aatgaagatg ctcaggaaag tgactctctg
     2161 aaaactcatt tggaagatga aagagataat agcgaaaaga agaagaattt ggaagaagct
     2221 gaattacatt caactgagaa agttccatgt attgaatttg atgatgatga ttatgatacg
     2281 gattttgttc caccttctcc agaagaaatt atttctgctt cttcttcctc ttcaagatgc
     2341 cttagtacgt taaaggacct tgacacatct gacagaagag aggatgttct tagcacatca
     2401 aaagatcttt tgtcaaaacc tgagaaaatg agtatgcagg agctgaatcc agaaaccagc
     2461 acagactgtg acgctagaca gataagttta cagcagcagc ttattcatgt gatggagcac
     2521 atctgtaaat taattgatac tattcctgat gataaactga aacttttgga ttgtgggaac
     2581 gaactgcttc agcagcggaa cataagaagg aaacttctaa cggaagtaga ttttaataaa
     2641 agtgatgcca gtcttcttgg ctcattgtgg agatacaggc ctgattcact tgatggccct
     2701 atggagggtg attcctgccc tacagggaat tctatgaagg agttaaattt ttcacacctt
     2761 ccctcaaatt ctgtttctcc tggggactgt ttactgacta ccaccctagg aaagacagga
     2821 ttctctgcca ccaggaagaa tctttttgaa aggcctttat tcaataccca tttacagaag
     2881 tcctttgtaa gtagcaactg ggctgaaaca ccaagactag gaaaaaaaaa tgaaagctct
     2941 tatttcccag gaaatgttct cacaagcact gctgtgaaag atcagaataa acatactgct
     3001 tcaataaatg acttagaaag agaaacccaa ccttcctatg atattgataa ttttgacata
     3061 gatgactttg atgatgatga tgactgggaa gacataatgc ataatttagc agccagcaaa
     3121 tcttccacag ctgcctatca acccatcaag gaaggtcggc caattaaatc agtatcagaa
     3181 agactttcct cagccaagac agactgtctt ccagtgtcat ctactgctca aaatataaac
     3241 ttctcagagt caattcagaa ttatactgac aagtcagcac aaaatttagc atccagaaat
     3301 ctgaaacatg agcgtttcca aagtcttagt tttcctcata caaaggaaat gatgaagatt
     3361 tttcataaaa aatttggcct gcataatttt agaactaatc agctagaggc gatcaatgct
     3421 gcactgcttg gtgaagactg ttttatcctg atgccgactg gaggtggtaa gagtttgtgt
     3481 taccagctcc ctgcctgtgt ttctcctggg gtcactgttg tcatttctcc cttgagatca
     3541 cttatcgtag atcaagtcca aaagctgact tccttggata ttccagctac atatctgaca
     3601 ggtgataaga ctgactcaga agctacaaat atttacctcc agttatcaaa aaaagaccca
     3661 atcataaaac ttctatatgt cactccagaa aagatctgtg caagtaacag actcatttct
     3721 actctggaga atctctatga gaggaagctc ttggcacgtt ttgttattga tgaagcacat
     3781 tgtgtcagtc agtggggaca tgattttcgt caagattaca aaagaatgaa tatgcttcgc
     3841 cagaagtttc cttctgttcc ggtgatggct cttacggcca cagctaatcc cagggtacag
     3901 aaggacatcc tgactcagct gaagattctc agacctcagg tgtttagcat gagctttaac
     3961 agacataatc tgaaatacta tgtattaccg aaaaagccta aaaaggtggc atttgattgc
     4021 ctagaatgga tcagaaagca ccacccatat gattcaggga taatttactg cctctccagg
     4081 cgagaatgtg acaccatggc tgacacgtta cagagagatg ggctcgctgc tcttgcttac
     4141 catgctggcc tcagtgattc tgccagagat gaagtgcagc agaagtggat taatcaggat
     4201 ggctgtcagg ttatctgtgc tacaattgca tttggaatgg ggattgacaa accggacgtg
     4261 cgatttgtga ttcatgcatc tctccctaaa tctgtggagg gttactacca agaatctggc
     4321 agagctggaa gagatgggga aatatctcac tgcctgcttt tctataccta tcatgatgtg
     4381 accagactga aaagacttat aatgatggaa aaagatggaa accatcatac aagagaaact
     4441 cacttcaata atttgtatag catggtacat tactgtgaaa atataacgga atgcaggaga
     4501 atacagcttt tggcctactt tggtgaaaat ggatttaatc ctgatttttg taagaaacac
     4561 ccagatgttt cttgtgataa ttgctgtaaa acaaaggatt ataaaacaag agatgtgact
     4621 gacgatgtga aaagtattgt aagatttgtt caagaacata gttcatcaca aggaatgaga
     4681 aatataaaac atgtaggtcc ttctggaaga tttactatga atatgctggt cgacattttc
     4741 ttggggagta agagtgcaaa aatccagtca ggtatatttg gaaaaggatc tgcttattca
     4801 cgacacaatg ccgaaagact ttttaaaaag ctgatacttg acaagatttt ggatgaagac
     4861 ttatatatca atgccaatga ccaggcgatc gcttatgtga tgctcggaaa taaagcccaa
     4921 actgtactaa atggcaattt aaaggtagac tttatggaaa cagaaaattc cagcagtgtg
     4981 aaaaaacaaa aagcgttagt agcaaaagtg tctcagaggg aagagatggt taaaaaatgt
     5041 cttggagaac ttacagaagt ctgcaaatct ctggggaaag tttttggtgt ccattacttc
     5101 aatattttta ataccgtcac tctcaagaag cttgcagaat ctttatcttc tgatcctgag
     5161 gttttgcttc aaattgatgg tgttactgaa gacaaactgg aaaaatatgg tgcggaagtg
     5221 atttcagtat tacagaaata ctctgaatgg acatcgccag ctgaagacag ttccccaggg
     5281 ataagcctgt ccagcagcag aggccccgga agaagtgccg ctgaggagct cgacgaggaa
     5341 atacccgtat cttcccacta ctttgcaagt aaaaccagaa atgaaaggaa gaggaaaaag
     5401 atgccagcct cccaaaggtc taagaggaga aaaactgctt ccagtggttc caaggcaaag
     5461 ggggggtctg ccacatgtag aaagatatct tccaaaacga aatcctccag catcattgga
     5521 tccagttcag cctcacatac ttctcaagcg acatcaggag ccaatagcaa attggggatt
     5581 atggctccac cgaagcctat aaatagaccg tttcttaagc cttcatatgc attctcataa
     5641 caaccgaatc tcaatgtaca tagaccctct ttcttgtttg tcagcatctg accatctgtg
     5701 actataaagc tgttattctt gttataccaa aaaaaaaaaa aaaaaaaaac tcgacggtac
     5761 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     5821 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     5881 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     5941 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6001 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     6061 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     6121 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     6181 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     6241 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     6301 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6361 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6421 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6481 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6601 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     6661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6721 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6781 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6841 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6901 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6961 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     7021 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     7081 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     7141 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     7201 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     7261 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     7321 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     7381 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     7441 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     7501 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     7561 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     7621 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     7681 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     7741 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     7801 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     7861 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7921 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     7981 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     8041 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     8101 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     8161 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     8221 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     8281 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8341 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8401 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8461 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8521 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     8581 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8641 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8701 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8761 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8821 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8881 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8941 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9001 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
