LOCUS       sequence-150379-         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding two shRNAs and mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pFUChW Parkin shRNA Mul1 shRNA
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(60..770)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(338..357)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     607..625
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      767..776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(883..902)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 tctttcacaa attttgtaat ccagaggttg attatcgata agcttgatat cgaattgctt
       61 tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt
      121 tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt gacgttgtag
      181 gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc agcgtcgtag
      241 tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc gccgtcctcg
      301 gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat tacggggccg
      361 tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc gtcctgcagg
      421 gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac gcgctcccac
      481 ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg gtgcttcacg
      541 taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa gggcaggggg
      601 ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
      661 tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac cttgaagcgc
      721 atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat ggtggcgacc
      781 ggtactagtc taacactgaa aattacatat tgacccaaat gattacattt caaaaggtgc
      841 ctaaaaaact tcacaaaaca cactcgccaa ccccgagcgc atagttcaaa accggagctt
      901 cagctactta agaagatagg tacataaa
//
