LOCUS       sequence-150424-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NLS-Cas9-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3081"; 
            journalName: "Nat Biotechnol"; date: "2015-01"; volume: "33"; issue:
            "1"; pages: "73-80"
COMMENT     Sequence Label: pET-NLS-Cas9-6xHis
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    68..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             107..129
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             139..159
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatcg atctcgatcc cgcgaaatta atacgactca
       61 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
      121 agaaggagat ataccatgcc caagaagaag aggaaggtga tggataagaa atactcaata
      181 ggcttagata tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
      241 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
      301 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
      361 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
      421 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
      481 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
      541 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
      601 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
      661 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
      721 ttggtacaaa cctacaatca attatttgaa gaa
//
