LOCUS       sequence-150429-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses  CrTRP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Arias-Darraz L, Cabezas D, Colenso CK, Alegria-Arcos M, Bravo-Moraga
            F, Varas-Concha I, Almonacid DE, Madrid R, Brauchi S
  TITLE     A transient receptor potential ion channel in Chlamydomonas shares 
            key features with sensory transduction-associated TRP channels in 
            mammals.
  JOURNAL   Plant Cell. 2015 Jan;27(1):177-88. doi: 10.1105/tpc.114.131862. Epub
            2015 Jan 16.
  PUBMED    25595824
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.114.131862"; journalName: "Plant Cell"; date: 
            "2015-01"; volume: "27"; issue: "1"; pages: "177-88"
COMMENT     Sequence Label: pTracer_Cr-TRP1
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(112..114,115..117,118..828)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(112..114,115..117,118..828)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(157..178)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(418..437)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     765..786
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gatggtgagc
      121 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      181 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      241 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      301 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
      361 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      421 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
      481 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
      541 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
      601 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
      661 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
      721 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
      781 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg cgcaggtgcc
      841 ggggccaagc ttggtaccag cgacatgaag gtcgctccag cgccagcctc agggcagcct
      901 ggcggaggca gcaagaagcc atcggactat gg
//
