LOCUS       sequence-150482-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driven flipped + extended sgRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Waaijers S, Munoz J, Berends C, Ramalho JJ, Goerdayal SS, Low TY, 
            Zoumaro-Djayoon AD, Hoffmann M, Koorman T, Tas RP, Harterink M, 
            Seelk S, Kerver J, Hoogenraad CC, Bossinger O, Tursun B, van den 
            Heuvel S, Heck AJ, Boxem M
  TITLE     A tissue-specific protein purification approach in Caenorhabditis 
            elegans identifies novel interaction partners of DLG-1/Discs large.
  JOURNAL   BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x.
  PUBMED    27506200
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2016-08-9"; volume: "14:66. doi"; pages: " 
            10.1186/s12915-016-0286-x"
COMMENT     Sequence Label: pJJR50
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..366
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     complement(506..522)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(506..522)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(559..577)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(598..614)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(598..614)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(611..633)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    622..638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(646..652,653..670,671..676))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    691..712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(829..846)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatcgataag cttgatcaaa aaaaactagc aataaaggaa taaaaaactg tacaccttaa
       61 aggcgcacac tctgttttgc aaattttatt tttagttgtg aattttctgc tgagacctga
      121 aaatagcaac tttagtacta ctataatttg tcaacctttt caaaaaaagc atgcaatttt
      181 tgagaaactc ttataaaagc tattattaaa aaaacacctt ttttccaaaa ttattccaca
      241 aaaaatatgt tatgaaatgc ctacaccctc tcacacacac tctttatact actctgtcaa
      301 actcacgaga tgtctgccgc ctcttgtgtt gcccctatat aaacacctcc tattgcgaga
      361 tgtcttatgt cttcgagaga ggaagactag tttaagagct atgctggaaa cagcatagca
      421 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      481 tttatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg
      541 gagctccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
      601 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      661 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      721 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      781 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      841 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      901 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      961 aaa
//
