LOCUS       sequence-150501-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of ZCD2 with a C-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Amr S, Heisey C, Zhang M, Xia XJ, Shows KH, Ajlouni K, Pandya A, 
            Satin LS, El-Shanti H, Shiang R
  TITLE     A homozygous mutation in a novel zinc-finger protein, ERIS, is 
            responsible for Wolfram syndrome 2.
  JOURNAL   Am J Hum Genet. 2007 Oct;81(4):673-83. Epub 2007 Aug 20.
  PUBMED    17846994
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet."; date: "2007-10"; volume: "81(4)"; pages: "673-83. Epub 2007
            Aug 20"
COMMENT     Sequence Label: pC-FLAG-ZCD2
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..129
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    139..163
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(689..713)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             715..738
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(758..777)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(759..777)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(788..805)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(788..804)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(797..819)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccggact ctagcctagg ccgcggacca tggcgtacta ccatcaccat
      121 caccatcact ctagatcaac aagtttgtac aaaaaagcag gctctttaaa ggaaccaatt
      181 cagtcgactg gatccggtac cgaattcccg ggatatcgtc gacccacgcg tccgggctcg
      241 ggagaggagt ggacgccgct ggccaggatg gtgctggaga gcgtggcccg tatcgtgaag
      301 gtgcagctcc ctgcatatct gaagcggctc ccagtccctg aaagcattac cgggttcgct
      361 aggctcacag tttcagaatg gcttcggtta ttgcctttcc ttggtgtact cgcacttctt
      421 ggctaccttg cagttcgtcc attcctcccg aagaagaaac aacagaagga tagcttgatt
      481 aatcttaaaa tacaaaagga aaatccgaaa gtagtgaatg aaataaacat tgaagatttg
      541 tgtcttacta aagcagctta ttgtaggtgt tggcgttcta aaacgtttcc tgcctgcgat
      601 ggttcacata ataaacacaa tgaattgaca ggagataatg tgggtccact aatactgaag
      661 aagaaagaag tagcctcgag atatctagac ccagctttct tgtacaaagt ggttgactac
      721 aaagacgatg acgacaagtg agacgtcata gctctctccc tatagtgagt cgtattataa
      781 gctaggcact ggccgtcgtt ttacaacgtc gtgactggga aa
//
