LOCUS       sequence-150515-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpyTagged GlpG protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Min D, Arbing MA, Jefferson RE, Bowie JU
  TITLE     A simple DNA handle attachment method for single molecule mechanical
            manipulation experiments.
  JOURNAL   Protein Sci. 2016 Aug;25(8):1535-44. doi: 10.1002/pro.2952. Epub 
            2016 Jun 6.
  PUBMED    27222403
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2952"; 
            journalName: "Protein Sci"; date: "2016-08"; volume: "25"; issue: 
            "8"; pages: "1535-44"
COMMENT     Sequence Label: pSpyTag-GlpG
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             101..127
                     /codon_start=1
                     /product="synthetic cistron containing a ribosome binding 
                     site (Shine-Dalgarno sequence), for enhancing the bacterial
                     expression of a downstream cistron (Schoner, 1997)"
                     /label=minicistron
                     /note="This first cistron should terminate 3 bp upstream of
                     the ATG for the second cistron."
                     /translation="MYRLNKEE"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             161..199
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             812..850
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
ORIGIN
        1 agcggcactg ctctttaaca atttatcaga caatctgtgt gggcactcga ccggaattat
       61 cgattaactt tattattaaa aattaaagag gtatatatta atgtatcgat taaataagga
      121 ggaataaacc atggggcatc atcatcatca tcatgagctc gctcatattg taatggtcga
      181 tgcgtataaa ccgacgaaag gttcaggaga gtcaggcgcc gcctgtttgc gtgaacgcgc
      241 aggtccggta acctgggtga tgatgatcgc cgccgtggtg gtgtttattg ccatgcaaat
      301 tctcggcgat caggaagtga tgttatggct ggcctggcca ttcgatccaa cactgaaatt
      361 tgagttctgg cgttacttca cccacgcgtt aatgcacttc tcgctgatgc atatcctctt
      421 taacctgctc tggtggtggt atctcggcgg tgcggtggaa aaacgcctcg gtagcggtaa
      481 gctaattgtc attacgctta tcagcgccct gttaagcggc tatgtgcagc aaaaattcag
      541 cgggccgtgg tttggcgggc tttctggcgt ggtgtatgcg ctgatgggct acgtctggct
      601 acgtggcgaa cgcgatccgc aaagtggcat ttacctgcaa cgtgggttaa ttatctttgc
      661 gctgatctgg attgtcgccg gatggtttga tttgtttggg atgtcgatgg cgaacggagc
      721 acacatcgcc gggttagccg tgggtttagc gatggctttt gttgattcgc tcaatgcgcg
      781 aaaacgaaaa tgtggtagtg gtgaaagtgg tgcccacatc gtgatggtgg acgcctacaa
      841 gccgacgaag gagtaagctt
//
