LOCUS       sequence-150655-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Bush TG, Savidge TC, Freeman TC, Cox HJ, Campbell EA, Mucke L, 
            Johnson MH, Sofroniew MV
  TITLE     Fulminant jejuno-ileitis following ablation of enteric glia in adult
            transgenic mice.
  JOURNAL   Cell. 1998 Apr 17. 93(2):189-201.
  PUBMED    9568712
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1998 
            Apr 17. 93(2):189-201."
COMMENT     Sequence Label: GFAP-HSV-tk (pTGB008)
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    33..167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(57..76)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     111..130
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     557..579
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     571..588
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     572..588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     599..618
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        599..617
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    650..674
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccgcgcgttg gccgattcat taatgcagga tcgatccaga catgataaga tacattgatg
       61 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      121 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
      181 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
      241 acctctacaa atgtggtatg gctgattatg atcatgaaca gactgtgagg actgaggggc
      301 ctgaaatgag ccttgggact gtgaatctaa aatacacaaa caattagaat cactagctcc
      361 tgtgtataat attttcataa atcatactca gtaagcaaaa ctctcaagca gcaagcatat
      421 gcagctagtt taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc
      481 tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc
      541 ccaagctagc agttttccca gtcacgacgt tgtaaaacga cggccagtgc ctagcttata
      601 atacgactca ctatagggag agagctatga cgtcgcatgc acgcgatcaa ccactttgta
      661 caagaaagct gggtctagat atctcgagtg cggccgcaat ctagagtcga g
//
