LOCUS       sequence-150713-        7274 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CCDC22 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7274)
  AUTHORS   Starokadomskyy P, Gluck N, Li H, Chen B, Wallis M, Maine GN, Mao X, 
            Zaidi IW, Hein MY, McDonald FJ, Lenzner S, Zecha A, Ropers HH, Kuss 
            AW, McGaughran J, Gecz J, Burstein E
  TITLE     CCDC22 deficiency in humans blunts activation of proinflammatory 
            NF-kappaB signaling.
  JOURNAL   J Clin Invest. 2013 May;123(5):2244-56. doi: 10.1172/JCI66466. Epub 
            2013 Apr 8.
  PUBMED    23563313
REFERENCE   2  (bases 1 to 7274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1172/JCI66466"; 
            journalName: "J Clin Invest"; date: "2013-05"; volume: "123"; issue:
            "5"; pages: "2244-56"
COMMENT     Sequence Label: pEBB HA CCDC22
FEATURES             Location/Qualifiers
     source          1..7274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        252..462
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        274..470
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        557..1739
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          788..1730
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1687..1707
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1804..1830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1810..1836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(4408..4425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4408..4424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4417..4439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4637..5092
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4724..4743)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4934..4955
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5109..5128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5228..5250
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5288..5306)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5374..5478
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5479..5547,5548..6339)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5697..5716)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6510..7098
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6999..7018
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7252..7269
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttc
      241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctttgca
      481 aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt
      541 gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      601 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
      661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
      841 ttgcgtgcct tgaattactt ccacgcccct ggctgcagta cgtgattctt gatcccgagc
      901 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
      961 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     1021 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     1081 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     1141 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     1201 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     1261 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     1321 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     1381 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     1441 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     1501 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     1561 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     1621 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     1681 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     1741 gaattctcta gagatccctc gacctcgaga tccattgtgc tggatctcac catggcttct
     1801 agctatcctt atgacgtgcc tgactatgcc agcctgggat ccatggagga ggcggaccga
     1861 atcctcatcc attcgctgcg ccaggccggc acggcagttc ctccagatgt gcagaccttg
     1921 cgcgccttca ccactgagct ggttgtagag gctgtggtcc gctgcctgcg tgtgatcaac
     1981 cctgcggtgg gctctggcct cagccctctg ctgcctcttg ccatgtctgc ccggttccgc
     2041 ctggccatga gcctggctca ggcctgcatg gacctgggct atcccttgga gcttggctat
     2101 cagaacttcc tctaccccag tgagcctgac ctccgagacc tgcttctctt cttggctgag
     2161 cgtctgccca ccgatgcctc tgaggatgca gaccagcctg caggtgactc agctattctc
     2221 ctccgggcca ttgggagcca aattcgggac cagctggcac tgccttgggt cccgccccac
     2281 cttcgcactc ccaagctgca gcacctccag ggctcggccc tccagaagcc tttccatgcc
     2341 agcaggctgg tcgtgccaga attgagttcc agaggtgagc cacgggagtt ccaggcgagt
     2401 cccctgctgc ttccagtccc tacccaggtg cctcagcctg ttggaagggt ggcctcgctc
     2461 ctcgaacacc atgccctgca gctctgccag cagacgggcc gggaccggcc aggggatgag
     2521 gactgggtcc accggacatc ccgcctccca ccccaggagg acacacgggc tcagcggcag
     2581 cggctgcaga agcaactgac tgagcatctg cgccaaagct ggggcctgct tggggccccc
     2641 atacaagccc gggacctggg agaactgctg caggcctggg gtgctggggc caagactggt
     2701 gctcctaagg gctcccgctt cacgcactca gagaagttca ccttccatct ggagccccag
     2761 gcccaggcca ctcaggtgtc agatgtgcca gccacctccc ggcggcctga acaggtcacg
     2821 tgggcagctc aggaacagga gctcgagtcc cttcgggagc agctggaagg agtgaaccgc
     2881 agcattgagg aggttgaggc cgacatgaag accctgggcg tcagctttgt gcaggcagag
     2941 tctgagtgcc ggcacagcaa gctcagtaca gcagagcgtg agcaggccct gcgcctgaag
     3001 agccgcgcgg tggagctgct gcccgatggg actgccaacc ttgccaagct gcagcttgtg
     3061 gtggagaata gtgcccagcg ggtcatccac ttggcgggtc agtgggagaa gcaccgggtc
     3121 ccactcctcg ctgagtaccg ccacctccga aagctgcagg attgcagaga gctggaatct
     3181 tctcgacggc tggcagagat ccaagaactg caccagagtg tccgggcggc tgctgaagag
     3241 gcccgcagga aggaggaggt ctataagcag ctgatgtcag agctggagac tctgcccaga
     3301 gatgtgtccc ggctggccta cacccagcgc atcctggaga tcgtgggcaa catccggaag
     3361 cagaaggaag agatcaccaa gatcttgtct gatacgaagg agcttcagaa ggaaatcaac
     3421 tccctatctg ggaagctgga ccggacgttt gcggtgactg atgagcttgt gttcaaggat
     3481 gccaagaagg acgatgctgt tcggaaggcc tataagtatc tagctgctct gcacgagaac
     3541 tgcagccagc tcatccagac catcgaggac acaggcacca tcatgcggga ggttcgagac
     3601 ctcgaggagc agatcgagac agagctgggc aagaagaccc tcagcaacct ggagaagatc
     3661 cgggaggact accgagccct ccgccaggag aacgctggcc tcctaggccg ggtccgggag
     3721 gcctgagcgg ccgcgactct agagtgaggg tccccacctg ggacccttga gagtatcagg
     3781 tctcccacgt gggagacaag aaatccctgt ttaatattta aacagcagtg ttccccatct
     3841 gggtccttgc acccctcact ctggcctcag ccgactgcac agcggcccct gcatcccctt
     3901 ggctgtgagg cccctggaca agcagaggtg gccagagctg ggaggcatgg ccctggggtc
     3961 ccacgaattt gctggggaat ctcgtttttc ttcttaagac ttttgggaca tggtttgact
     4021 cccgaacatc accgacgcgt ctcctgtttt tctgggtggc ctcgggacac ctgccctgcc
     4081 cccacgaggg tcaggactgt gactcttttt agggccaggc aggtgcctgg acatttgcct
     4141 tgctggacgg ggactgggga tgtgggaggg agcagacagg aggaatcatg tcaggcctgt
     4201 gtgtgaaagg aagctccact gtcaccctcc acctcttcac cccccactca ccagtgtccc
     4261 ctccactgtc acattgtaac tgaacttcag gataataaag tgtttgcctc caaaaaaaaa
     4321 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     4381 aaaaaaaaaa aaaaaaaaaa agaattcact ggccgtcgtt ttacaacgtc gtgactggga
     4441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     4501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     4561 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     4621 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4681 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4741 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4801 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     4861 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4921 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     4981 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5041 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     5101 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     5161 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     5221 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     5281 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     5341 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5401 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5461 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5521 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5581 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5641 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5701 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5761 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5821 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5881 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5941 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6001 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6061 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6121 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6181 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6241 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6301 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6361 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6421 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6481 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6541 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6601 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6661 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6721 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6781 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6841 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6901 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6961 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7021 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7081 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7141 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7201 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7261 gtgagcgagg aagc
//
