LOCUS       sequence-150739-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  negative control for expression assays.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  TITLE     pTREtight reporter plasmids--Roderic Guigo
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTre-Tight-luciferase-GFP (467)
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(95..97,98..100,101..811)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VSFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(140..161)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(401..420)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     748..769
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_RNA        956..974
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        995..1013
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
ORIGIN
        1 cgagctcggt acccggggat cctaatacga ctcactatag ggagacccaa gctggctagc
       61 gtttaaactt aagcttggta ccgagctcgg atccatggtg agcaagggcg aggagctgtt
      121 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      181 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      241 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      301 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      361 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      421 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      481 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      541 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgagct tcaagatccg
      601 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      661 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
      721 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      781 gatcactctc ggcatggacg agctgtacaa gggatccact agtccagtgt ggtggaattc
      841 tgcagatatc cagcacagtg gcggccgctc gctttcttgc tgtccaattt ctattaaagg
      901 ttcctttgtt ccctaagtcc aactaccaaa ctgggtctag ctctagctgt agaaaacatg
      961 aggatcaccc atgtctgctg gacgactgta gaaaacatga ggatcaccca tgtctgctgt
     1021 ctagctgtag aaaa
//
