LOCUS       sequence-150754-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Generates Alu inserts suitable for rescue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Wagstaff BJ, Hedges DJ, Derbes RS, Campos Sanchez R, Chiaromonte F, 
            Makova KD, Roy-Engel AM
  TITLE     Rescuing Alu: recovery of new inserts shows LINE-1 preserves Alu 
            activity through A-tail expansion.
  JOURNAL   PLoS Genet. 2012;8(8):e1002842. doi: 10.1371/journal.pgen.1002842. 
            Epub 2012 Aug 9.
  PUBMED    22912586
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2012"; volume: "8(8):e1002842. doi"; pages: " 
            10.1371/journal.pgen.1002842. Epub 2012 Aug 9"
COMMENT     Sequence Label: pBS AluYa5rescueA70D
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..155
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     complement(237..256)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcata
       61 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
      121 catcggcata gtatatcggc atagtataat acgacaaggt gaggaggacc accatgggat
      181 cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
      241 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
      301 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
      361 tcgagtactc caaacctaat caatatactt tcgcatacaa attaggtccc agcggctcca
      421 gtgttgcatc acttcatccg ctagctccca ttaaggagag gtccgactat atcttatgag
      481 aagaatacat cttccccgac cgacatttag tctgtgaact gcatccatat caacagaaga
      541 tctgttgact taggacttgg ctgcgtggtt aggaccatgt ccgtcagctt attaccatac
      601 cctttgcaag gccatctcaa agtttcccct gagacttggt actgaacggc tgttgacccc
      661 tttcccgcaa tttgacggtc ttgcctttta aaccgatgca atctattggt ttaaagacta
      721 gctaccaggt gcatgcctga taacttttcc ctgcaattca ttatattgct aattacagga
      781 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      841 cgttgtcact gaagcgggaa gggactggct gctattgggc ga
//
