LOCUS       sequence-150766-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Input plasmid: expresses three serine recombinases (TP901, BxbI, and
            A118) downstream of three inducible promoters..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Roquet N, Soleimany AP, Ferris AC, Aaronson S, Lu TK
  TITLE     Synthetic recombinase-based state machines in living cells.
  JOURNAL   Science. 2016 Jul 22;353(6297):aad8559. doi: 
            10.1126/science.aad8559. Epub 2016 Jul 21.
  PUBMED    27463678
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-07-22"; volume: "353(6297):aad8559. doi"; pages: " 
            10.1126/science.aad8559. Epub 2016 Jul 21"
COMMENT     Sequence Label: pNR220
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cacaggaaac ctactagatg aaacatcatc accatcacca ccaggccggc actaagaaag
       61 tagcaatcta tacacgagta tccactacta accaagcaga ggaaggcttc tcaattgatg
      121 agcaaattga ccgtttaaca aaatatgctg aagcaatggg gtggcaagta tctgatactt
      181 atactgatgc tggtttttca ggggccaaac ttgaacgccc agcaatgcaa agattaatca
      241 acgatatcga gaataaagct tttgatacag ttcttgtata taagctagac cgcctttcac
      301 gtagtgtaag agatactctt tatcttgtta aggatgtgtt cacaaaaaat aaaatagact
      361 ttatctcgct taatgaaagt attgatactt cttctgctat gggtagcttg tttctcacta
      421 ttctttctgc aattaatgag tttgaaagag agaatataaa agaacgcatg actatgggta
      481 aactagggcg agcgaaatct ggtaagtcta tgatgtggac taagacagct tttgggtatt
      541 accacaacag aaagacaggt atattagaaa ttgttccttt acaagctaca atagttgaac
      601 aaatattcac tgattattta tcaggaatat cacttacaaa attaagagat aaactcaatg
      661 aatctggaca catcggtaaa gatataccgt ggtcttatcg taccctaaga caaacacttg
      721 ataatccagt ttactgtggt tatatcaaat ttaaggacag cctatttgaa ggtatgcaca
      781 aaccaattat cccttatgag acttatttaa aagttcaaaa agagctagaa gaaagacaac
      841 agcagactta tgaaaga
//
