LOCUS       sequence-150800-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP
            output.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ramakrishnan P, Tabor JJ
  TITLE     Repurposing Synechocystis PCC6803 UirS-UirR as a UV-violet/green 
            photoreversible transcriptional regulatory tool in E. coli.
  JOURNAL   ACS Synth Biol. 2016 Apr 27.
  PUBMED    27120220
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Apr 27."
COMMENT     Sequence Label: pPR219.05
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             205..918
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      945..1016
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgttttttg cgccgacatc ataacggttc tggcaaatat tctgaaatga gctggttagc
       61 tagtcacagt ttccaagcaa gatccgggcg aaaaaataaa atcgatgtca aaaatcaact
      121 agtttgtcaa aaatcgacca ggacagggtt gggaattttt cctaggatgg gactgtcaga
      181 agtctcccct taaggataag aataatgcgt aaaggcgaag agctgttcac tggtgtcgtc
      241 cctattctgg tggaactgga tggtgatgtc aacggtcata agttttccgt gcgtggcgag
      301 ggtgaaggtg acgcaactaa tggtaaactg acgctgaagt tcatctgtac tactggtaaa
      361 ctgccggtac cttggccgac tctggtaacg acgctgactt atggtgttca gtgctttgct
      421 cgttatccgg accatatgaa gcagcatgac ttcttcaagt ccgccatgcc ggaaggctat
      481 gtgcaggaac gcacgatttc ctttaaggat gacggcacgt acaaaacgcg tgcggaagtg
      541 aaatttgaag gcgataccct ggtaaaccgc attgagctga aaggcattga ctttaaagaa
      601 gacggcaata tcctgggcca taagctggaa tacaatttta acagccacaa tgtttacatc
      661 accgccgata aacaaaaaaa tggcattaaa gcgaatttta aaattcgcca caacgtggag
      721 gatggcagcg tgcagctggc tgatcactac cagcaaaaca ctccaatcgg tgatggtcct
      781 gttctgctgc cagacaatca ctatctgagc acgcaaagcg ttctgtctaa agatccgaac
      841 gagaaacgcg atcatatggt tctgctggag ttcgtaaccg cagcgggcat cacgcatggt
      901 atggatgaac tgtacaaatg atgataataa tctagaccag gcatcaaata aaacgaaagg
      961 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctc
//
