LOCUS       sequence-151017-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE
  TITLE     A UbcH5/ubiquitin noncovalent complex is required for processive 
            BRCA1-directed ubiquitination.
  JOURNAL   Mol Cell. 2006 Mar 17. 21(6):873-80.
  PUBMED    16543155
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Mar 17. 21(6):873-80."
COMMENT     Sequence Label: Ubiquitin WT
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             31..258
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             285..302
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             312..329
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(401..419)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      415..462
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(637..642,643..659,660..665))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(692..710)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagaggatcc atgcagatct tcgtcaagac gttaaccggt
       61 aaaaccataa ctctggaagt tgaaccatcc gataccatcg aaaacgttaa ggctaaaatt
      121 caagacaagg aaggaattcc acctgatcaa caaagattga tctttgccgg taagcagctc
      181 gaggacggta gaacgctgtc tgattacaac attcagaagg agtcgacctt acatcttgtc
      241 ttaagactaa gaggtggttg atgaggtacc ccatgggcag cagccatcat catcatcatc
      301 acagcagcgg cctggtgccg cgcggcagcc atatgctcga ggatcgtcga cgatccggct
      361 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      421 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      481 tccggatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat
      541 ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct
      601 gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct
      661 gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      721 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      781 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      841 taaccctgat aaatgcttca ataatattga aaaaggaaag agtatgagta ttcaacattt
      901 ccgtgtcgcc cttattccct
//
