LOCUS       sequence-151040-        8717 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression plasmid for kinase dead Plk4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Moyer TC, Clutario KM, Lambrus BG, Daggubati V, Holland AJ
  TITLE     Binding of STIL to Plk4 activates kinase activity to promote 
            centriole assembly.
  JOURNAL   J Cell Biol. 2015 Jun 22;209(6):863-78. doi: 10.1083/jcb.201502088.
  PUBMED    26101219
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2015-06-22- 22"; volume: "209"; 
            issue: "6"; pages: "863-78"
COMMENT     Sequence Label: pcDNA5 FRT/TO Plk4 KD-mCherry
FEATURES             Location/Qualifiers
     source          1..8717
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      993..1002
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2729..2738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3924..4634
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4069..4087)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4337..4356
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4669..4686)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4675..4899
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5032..5051)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5183..5230
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5238..6260
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5238..6260
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6390..6511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6427..6446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6481..6500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6560..6576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6560..6576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6573..6595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6584..6600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6608..6614,6615..6632,6633..6638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6653..6674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6791..6808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6962..7550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7042..7061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7721..8512,8513..8581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8344..8363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8582..8686)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttaccggtg ccgccaccat ggcgacctgc atcggggaga
     1021 agatcgagga ttttaaagtt ggaaatctgc ttggtaaagg atcatttgct ggtgtctaca
     1081 gagctgagtc cattcacact ggtttggaag ttgcaatcaa aatgatagat aagaaagcca
     1141 tgtacaaagc aggaatggta cagagagtcc aaaatgaggt gaaaatacat tgccaattga
     1201 aacatccttc tatcttggag ctttataact attttgaaga tagcaattat gtgtatctgg
     1261 tattagaaat gtgccataat ggagaaatga acaggtatct aaagaataga gtgaaaccct
     1321 tctcagaaaa tgaagctcga cacttcatgc accagatcat cacagggatg ttgtatcttc
     1381 attctcatgg tatactacac cgggacctca cactttctaa cctcctactg actcgtaata
     1441 tgaacatcaa gattgctgct tttgggctgg caactcaact gaaaatgcca catgaaaagc
     1501 actatacatt atgtggaact cctaactaca tttcaccaga aattgccact cgaagtgcac
     1561 atggccttga atctgatgtt tggtccctgg gctgtatgtt ttatacatta cttatcggga
     1621 gaccaccctt cgacactgac acagtcaaga acacattaaa taaagtagta ttggcagatt
     1681 atgaaatgcc atcttttttg tcaatagagg ccaaggacct tattcaccag ttacttcgta
     1741 gaaatccagc agatcgttta agtctgtctt cagtattgga ccatcctttt atgtcccgaa
     1801 attcttcaac aaaaagtaaa gatttaggaa ctgtggaaga ctcaattgat agtgggcatg
     1861 ccacaatttc tactgcaatt acagcttctt ccagtaccag tataagtggt agtttatttg
     1921 acaaaagaag acttttgatt ggtcagccac tcccaaataa aatgactgta tttccaaaga
     1981 ataaaagttc aactgatttt tcttcttcag gagatggaaa cagtttttat actcagtggg
     2041 gaaatcaaga aaccagtaat agtggaaggg gaagagtaat tcaagatgca gaagaaaggc
     2101 cacattctcg ataccttcgt agagcttatt cctctgatag atctggcact tctaatagtc
     2161 agtctcaagc aaaaacatat acaatggaac gatgtcactc agcagaaatg ctttcagtgt
     2221 ccaaaagatc aggaggaggt gaaaatgaag agaggtactc acccacagac aacaatgcca
     2281 acatttttaa cttctttaaa gaaaagacat ccagtagttc tggatctttt gaaagacctg
     2341 ataacaatca agcactctcc aatcatcttt gtccaggaaa aactcctttt ccatttgcag
     2401 acccgacacc tcagactgaa accgtacaac agtggtttgg gaatctgcaa ataaatgctc
     2461 atttaagaaa aactactgaa tatgacagca tcagcccaaa ccgggacttc cagggccatc
     2521 cagatttgca gaaggacaca tcaaaaaatg cctggactga tacaaaagtc aaaaagaact
     2581 ctgatgcttc tgataatgca cattctgtaa aacagcaaaa taccatgaaa tatatgactg
     2641 cacttcacag taaacctgag ataatccaac aagaatgtgt ttttggctca gatcctcttt
     2701 ctgaacagag caagactagg ggtatggagc caccatgggg ttatcagaat cgtacattaa
     2761 gaagcattac atctccgttg gttgctcaca ggttaaaacc aatcagacag aaaaccaaaa
     2821 aggctgtggt gagcatactt gattcagagg aggtgtgtgt ggagcttgta aaggagtatg
     2881 catctcaaga atatgtgaaa gaagttcttc agatatctag tgatggaaat acgatcacta
     2941 tttattatcc aaatggtggt agaggttttc ctcttgctga tagaccaccc tcacctactg
     3001 acaacatcag taggtacagc tttgacaatt taccagaaaa atactggcga aaatatcaat
     3061 atgcttccag gtttgtacag cttgtaagat ctaaatctcc caaaatcact tattttacaa
     3121 gatatgctaa atgcattttg atggagaatt ctcctggtgc tgattttgag gtttggtttt
     3181 atgatggggt aaaaatacac aaaacagaag atttcattca ggtgattgaa aagacaggga
     3241 agtcttacac tttaaaaagt gaaagtgaag ttaatagctt gaaagaggag ataaaaatgt
     3301 atatggacca tgctaatgag ggtcatcgta tttgtttagc actggaatcc ataatatcag
     3361 aagaggaaag gaaaactagg agtgctccct ttttcccaat aatcatagga agaaaacctg
     3421 gtagtactag ttcacctaag gccttatcac ctcctccttc tgtggattca aattacccaa
     3481 cgagagagag agcatctttc aacagaatgg tcatgcatag tgctgcttct ccaacacagg
     3541 caccaatcct taatccctct atggttacaa atgaaggact tggtcttaca actacagctt
     3601 ctggaacaga catctcttct aatagtctaa aagattgtct tcctaaatca gcacaacttt
     3661 tgaaatctgt ttttgtgaaa aatgttggtt gggctacaca gttaactagt ggagctgtgt
     3721 gggttcagtt taatgatggg tcccagttgg ttgtgcaggc aggagtgtct tctatcagtt
     3781 atacctcacc aaatggtcaa acaactaggt atggagaaaa tgaaaaatta ccagactaca
     3841 tcaaacagaa attacagtgt ctgtcttcca tccttttgat gttttctaat ccgactccta
     3901 attttcatcc cggggcggat ccaatggtga gcaagggcga ggaggataac atggccatca
     3961 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     4021 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     4081 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     4141 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     4201 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     4261 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     4321 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     4381 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     4441 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     4501 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     4561 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     4621 agctgtacaa gtaaagcggc cgcgggcccg tttaaacccg ctgatcagcc tcgactgtgc
     4681 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     4741 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     4801 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     4861 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     4921 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4981 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5041 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5101 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5161 agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta
     5221 taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     5281 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     5341 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     5401 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     5461 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     5521 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     5581 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     5641 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     5701 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     5761 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     5821 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     5881 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     5941 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     6001 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     6061 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     6121 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     6181 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     6241 gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc gccttctatg
     6301 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6361 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6541 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8701 aagtgccacc tgacgtc
//
