LOCUS       sequence-151215-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector encoding two shRNAs and mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Rojansky R, Cha MY, Chan DC
  TITLE     Elimination of paternal mitochondria in mouse embryos occurs through
            autophagic degradation dependent on PARKIN and MUL1.
  JOURNAL   Elife. 2016 Nov 17;5. pii: e17896. doi: 10.7554/eLife.17896.
  PUBMED    27852436
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-11-17"; volume: "5. pii: e17896. doi"; pages: " 
            10.7554/eLife.17896"
COMMENT     Sequence Label: pFUChW Parkin shRNA Mul1 shRNA
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    120..237
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(354..567)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(651..741)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
ORIGIN
        1 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
       61 tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat
      121 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
      181 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      241 gtttattaca gggacagcag agatccagtt tggttaatta agaattggct agcaaaaaga
      301 gctgtgcggt ctgttaatct cttgaattaa cagaccgcac agctctctag aacgtggtct
      361 catacagaac ttataagatt cccaaatcca aagacatttc acgtttatgg tgatttccca
      421 gaacacatag cgacatgcaa atattgcagg gcgccactcc cctgtccctc acagccatct
      481 tcctgccagg gcgcacgcgc gctgggtgtt cccgcctagt gacactgggc ccgcgattcc
      541 ttggagcggg ttgatgacgt cagcgtttct cggtgctggg ctccgaattc aaaaagagga
      601 gaagtcatga aacatctctt gaatgtttca tgacttctcc tcggatccct gtggtctcat
      661 acagaactta taagattccc aaatccaaag acatttcacg tttatggtga tttcccagaa
      721 cacatagcga catgcaaata ttgcagggcg ccactcccct gtccctcaca gccatcttcc
      781 tgccagggcg cacgcgcgct gggtgttccc gcctagtgac actgggcccg cgattccttg
      841 gagcgggttg atgacgtc
//
