LOCUS       sequence-151259-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Indicator of caspase-3-like protease activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Zhang J, Wang X, Cui W, Wang W, Zhang H, Liu L, Zhang Z, Li Z, Ying 
            G, Zhang N, Li B
  TITLE     Visualization of caspase-3-like activity in cells using a 
            genetically encoded fluorescent biosensor activated by protein 
            cleavage.
  JOURNAL   Nat Commun. 2013;4:2157. doi: 10.1038/ncomms3157.
  PUBMED    23857461
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2157. doi"; pages: " 
            10.1038/ncomms3157"
COMMENT     Sequence Label: pCDH-puro-CMV-CC3AI
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             5..273
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(286..497)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(542..571)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cttggatctg taacggcgca gaacagaaaa cgaaacaaag acgtagagtt gagcaagcag
       61 ggtcaggcaa agcgtggaga gccggctgag tctaggtagg ctccaaggga gcgccggaca
      121 aaggcccggt ctcgacctga gctttaaact tacctagacg gcggacgcag ttcaggaggc
      181 accacaggcg ggaggcggca gaacgcgact caaccggcgt ggatggcggc ctcaggtagg
      241 gcggcgggcg cgtgaaggag agatgcgagc ccctcgaagc ttcagctgtg ttctggcggc
      301 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
      361 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
      421 ccttctctag gcacccgttc aattgccgac ccctcccccc aacttctcgg ggactgtggg
      481 cgatgtgcgc tctgcccact gacgggcacc ggagcgatcg cagatccttc gcggccgctt
      541 acaggtcctc ctcgctgatc agcttctgct cgttaggcag gttgtccacc ctcatcaggt
      601 ccagctccct ctcgaagatc tcgtcgatag gcagcatctg gccgtccacg gtcatgaact
      661 tgtggtcctt ggtggccctg atcaggctgc cgtcctccag gcagtactcg aacacctcct
      721 gctcgcccct gtcgtgccac tgggccacag gctgggtgta gatgttgccg ttgttgtcca
      781 cgctgtacac ggtgcactcg atcctcttct ccacgatctt gccgataggc agcaggccgt
      841 actccacggt caggatctcg gtctcgtagc tcaggcagta ctcggcggat ccggcggtga
      901 tatagacgtt gtcgctgatg gcgttgtact ccagcttgtg ccccaggatg ttgccgtcct
      961 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     1021 cctcggcgcg ggtct
//
