LOCUS       sequence-151265-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Staphylococcus aureus MscL with C Terminal Truncation of 26 amino 
            acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Liu Z, Gandhi CS, Rees DC
  TITLE     Structure of a tetrameric MscL in an expanded intermediate state.
  JOURNAL   Nature. 2009 Sep 3;461(7260):120-4. doi: 10.1038/nature08277. Epub 
            2009 Aug 23.
  PUBMED    19701184
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature08277"; journalName: "Nature"; date: "2009-09-3- 3"; 
            volume: "461"; issue: "7260"; pages: "120-4"
COMMENT     Sequence Label: SaMscL (CDelta26)
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     279..298
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     382..401
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        382..400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    401..425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             440..462
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             508..525
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(942..960)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
       61 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
      121 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
      181 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
      241 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
      301 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
      361 gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
      421 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc
      481 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gttaaaagaa
      541 ttcaaagagt tcgccttaaa aggtaacgtc ttagatttag caattgctgt tgtgatgggt
      601 gcagctttca acaagattat atcttcatta gtagaaaata tcatcatgcc attaattggt
      661 aaaattttcg gatcagttga ttttgctaaa gaatggtcat tctggggtat taaatacggt
      721 ttatttatcc aatctgttat cgactttatt atcatcgcgt ttgctttatt catctttgtt
      781 aagattgcaa atacattaat gaagaaagaa taagccgaag aagaagcagt tgtggaagaa
      841 aatgttgtgt tattaactga aatcagagat ttattacgtg agaaaaaata aggatccggc
      901 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      961 ataacccctt ggggcctcta aacgggtctt gaggggtttt t
//
