LOCUS       sequence-151328-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transient and retroviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Srikanth S, Kim KD, Gao Y, Woo JS, Ghosh S, Calmettes G, Paz A, 
            Abramson J, Jiang M, Gwack Y
  TITLE     A large Rab GTPase encoded by CRACR2A is a component of subsynaptic 
            vesicles that transmit T cell activation signals.
  JOURNAL   Sci Signal. 2016 Mar 22;9(420):ra31. doi: 10.1126/scisignal.aac9171.
  PUBMED    27016526
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2016-03-22"; volume: "9(420):ra31. doi"; pages: " 
            10.1126/scisignal.aac9171"
COMMENT     Sequence Label: MO70-N-Flag-hCRACR2A-isoform-a
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     176..198
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     214..230
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             272..295
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
       61 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
      121 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
      181 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
      241 ggcgccggaa ttagatcgat ctctcgagat ggactacaaa gacgatgacg acaaggctgc
      301 ccctgacggg agggtagtct ccagacccca gagacttggt caggggtctg gccaggggcc
      361 aaaggggagt ggagcctgcc tgcatcccct ggacagcctg gagcagaagg agactcagga
      421 gcaaacgtcg ggccagctag tcatgctgag gaaggcacag gagttctttc agacctgtga
      481 tgctgaaggc aagggcttca tcgccaggaa ggatatgcag aggctgcata aggagctacc
      541 gctcagcctg gaggaactgg aggatgtgtt tgatgccctg gatgctgatg gcaatggcta
      601 tctgacccca caggagttca ctactggatt tagtcacttc ttcttcagcc agaataaccc
      661 aagtcaggaa gatgcaggtg aacaggtggc ccagcgccat gaagagaagg tgtatctgtc
      721 cagaggggat gaggatctgg gcgacatggg cgaagatgag gaagcccagt tccggatgct
      781 gatggacaga cttggagccc aaaaggtgtt ggaagatgaa agtgatgtca agcagctctg
      841 gttgcagctg aagaaggagg aacctcattt actgtccaac tttgaagact tcctgaccag
      901 aatcatctcc cagctccaag aagcccatga ggagaagaat gaactggagt gtgccctaaa
      961 aaggaaaa
//
