LOCUS       sequence-151345-         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (tse2) under the control of 
            rhamnose induceable promoter (PrhaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-246
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(322..568)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
ORIGIN
        1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
       61 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
      121 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
      181 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
      241 gctggagttc ttcgcccacc ccagcttcaa aagcgatcct ccgcggccca tccagtgcaa
      301 agctagcttc ttcgtctgtt tctactggta ttggcacaaa cctgattcca atttgagcaa
      361 ggctatgtgc catctcgata ctcgttctta actcaacaga agatgctttg tgcatacagc
      421 ccctcgttta ttatttatct cctcagccag ccgctgtgct ttcagtggat ttcggataac
      481 agaaaggccg ggaaataccc agcctcgctt tgtaacggag tagagacgaa agtgattgcg
      541 cctacccgga tattatcgtg aggatgcgtc atcgccatta attcactgat cagtgataag
      601 ctgtcaaaca tgagaattcg cggatcccta gagcccgcgg gctttttccc attggcggcg
      661 gatggcgttg cgcacgaagt tgtccagttg gcccatgagg acctcccggt acatcggctt
      721 ggccggcatg atggtgtagt gggtggcttg caggcgcttg ttgtagctgt cctgcttcac
      781 cttaaggtcc ggcgggatgt cggtgccgtc gggtatcacg aagtcgccgt cggcgcggcg
      841 cagcacgccg gcgcggtcga acaccgaggt gccgccggtg tccggggcgt a
//
