LOCUS       sequence-151358-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template plasmid which encodes kanamycin resistance gene for 
            positive selection and toxin gene (relE) under the control of 
            arabinose induceable promoter (ParaB) for negative selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
  TITLE     A set of powerful negative selection systems for unmodified 
            Enterobacteriaceae.
  JOURNAL   Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. 
            Epub 2015 Mar 23.
  PUBMED    25800749
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2015-07-27"; volume: "43(13):e83. doi"; pages: "
            10.1093/nar/gkv248. Epub 2015 Mar 23"
COMMENT     Sequence Label: pSLC-237
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      426..672
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     protein_bind    complement(725..758)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 acgcttttta tcgcaactct ctactgtttc tccatacctg caggtaccgg tccggaattc
       61 ttgacggagt aaagccatat ggcgtatttt ctggattttg acgagcgggc actaaaggaa
      121 tggcgaaagc tgggctcgac ggtacgtgaa cagttgaaaa agaagctggt tgaagtactt
      181 gagtcacccc ggattgaagc aaacaagctc cgtggtatgc ctgattgtta caagattaag
      241 ctccggtctt caggctatcg ccttgtatac caggttatag acgagaaagt tgtcgttttc
      301 gtgatttctg ttgggaaaag agaacgctcg gaagtatata gcgaggcggt caaacgcatt
      361 ctctgaggat ccgcgaattc tcatgtttga cagcttatca ctgatcagtg aattaatggc
      421 gatgacgcat cctcacgata atatccgggt aggcgcaatc actttcgtct ctactccgtt
      481 acaaagcgag gctgggtatt tcccggcctt tctgttatcc gaaatccact gaaagcacag
      541 cggctggctg aggagataaa taataaacga ggggctgtat gcacaaagca tcttctgttg
      601 agttaagaac gagtatcgag atggcacata gccttgctca aattggaatc aggtttgtgc
      661 caataccagt agaaacagac gaagaagcta gctttgcact ggatggggac gtcaggatcg
      721 ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact aaggaggata
      781 ttcatatgga ccatggctaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa
      841 ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa
      901 ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa
      961 aaccttagag g
//
