LOCUS       sequence-151393-        7182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human cTNT minigene with genomic segment containing alternative exon
            5 with CELF1 binding site mutated (CTGs->CAGs).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Philips AV, Timchenko LT, Cooper TA
  TITLE     Disruption of splicing regulated by a CUG-binding protein in 
            myotonic dystrophy.
  JOURNAL   Science. 1998 May 1;280(5364):737-41.
  PUBMED    9563950
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "1998-05-1"; volume: "280(5364)"; pages: "737-41"
COMMENT     Sequence Label: R300TA
FEATURES             Location/Qualifiers
     source          1..7182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..70)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        347..608
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4339..4358)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4340..4358)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4365..4382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4365..4381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4374..4396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4523..4978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4610..4629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4820..4841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5005..5109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5110..5178,5179..5970)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5328..5347)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6141..6729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6883..6900
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(7053..7058,7059..7076,7077..7083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7091..7107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7096..7118
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7115..7131
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7115..7131
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7152..7170
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtacatatc gaattcgagc tcgcccgggg atcatatggt gcactctcag tacaatctgc
       61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      241 atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
      301 agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
      361 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      421 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      481 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
      541 tattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc accacattgg
      601 tgtgcacctc caagctccgg actcgggtga gcccttatgc atgtctctta ctgaccagct
      661 tgctcttacc ggggttgcag ggaggcagat ttgctcacct gaatgttggg gaggtaaatg
      721 cttggagaga tgggggtgct gataaatcca tctctgtcct atgccggtca gagggtctgt
      781 gcatcgggag ctctccctct tcctttccca gctgccttct ttgcctttgg tttcatcctc
      841 acttggtttt atgctctgcc ttcgtctccc taactcctcc ctagtaggaa aaagcatcct
      901 ccgctgtggc accttgcaat tctccccatc tgcacccctc cctctgcctg ctgcgtgcag
      961 ccccttcctc cccaggttgc ccctcagcac ctgagtttcc gtaactcaag gactggagcc
     1021 cctgttactg accatacccc gctatggggc tgatctggca ttgctccccc acgccttttg
     1081 cgctttcagt gagggtgcac ggatatggga acgccagcaa aaatagctcc tgctcctcat
     1141 ccacctgtgg tggcttttgc ctcgcgtcgc cccaacctcc ctgagtcacg gggcctggct
     1201 gcgtctgagg agacagctgc agctccttgt gcagctcccc agccattttt agaagcactt
     1261 tccccacccc cctccccccc cctcttccca gcaatgtgtt gtgcccgcac attttccagg
     1321 ataaggtttc ctcggggagc ttggccctgc agcctttacc tcctcctcag gcaacgaccc
     1381 cgttaacatt ttgcctccca ggtctgcaga ttgctgtcta ttaggggggt tcacttctct
     1441 ataagggaat gccttcattt gggctcggcc ggtgctcctg ctggacccat tggtggctat
     1501 gagcaggaaa cactgctcta ataagtaaag ctgcatttat ggggaggaat tggcattgaa
     1561 aggtacgtac ctttccctga gaaatctctc catgcctttg ccaacttagc aatagccttc
     1621 agcactatgc ttcactgtct gtgctgagct ttctcccctg ccccatgcag tgcacacacg
     1681 tgctagcaat ccttgtcacc aatggtcact tcctgactct gttccctatg ccagaagcct
     1741 ccccagtctt tgggatgatt cttgtctatg gctgtgtcag tacagttttc cctctttagt
     1801 agacaacaag attaaagcct aaaatatctg atttcagttt gctacaggcc tatactatga
     1861 gggctgtatg aaagaacaac agatgtaaaa ttacttttat cctccttctt acttcattgt
     1921 gtgaagtggt acgtagttaa gccaaggaaa agacaagcct atagggagta ctgtcacaat
     1981 ctgtcttttt ccagccatgc accacttgtc ccagtgcacg tgtggctgtg agctggcaca
     2041 tggtttacct tttcatccac aaactgcagc ccagttccct ctgtgatgga aatttgaaga
     2101 agttgggaaa tatgagcaca gttgttgttc ctctttttgg tgtctagggg caatctggct
     2161 ttgttcttaa acccaagaaa gccaatgcta gcacgaataa ttgtgaatta ttttggttct
     2221 tgttgcaggt tcacaaccat ctaaagcaag atgtctgagt aagttcccat tttctgtctc
     2281 cactatgttg ctggtgttca accttttgtt cagcatcgat cctcactttg tagttcctct
     2341 gtaggcaaag cagttgtgga gtggtgggag gaaagaatgt cccatgactt ggctttcctg
     2401 atgccttctg gagaagaaag gaagggcgag caaaatccct ttcgcgaagg gcctttgctg
     2461 gcagttggat gctttccccc tttcctgctt ccctagccgg ccacgagatg gtgctgggaa
     2521 gcgaaagggt cgaccattgc agttaccaca gatcgcatgc tcctcttgtg ccaggcacct
     2581 gctgggcgct ctatgcacgc taggtcattc agtcctcacc acggctgaga agtgagtaga
     2641 atgattccca ttttactgat gaggcacctg aggctcaggg aagtaactga gagttgcccc
     2701 tgttgctgga aagtagtggc ttgggaattg gaatgccggc ctaactccaa agcccacact
     2761 cttaaaacca ctgcgctggg tggctgctcc tgccgcgggc tctctgctcc cagactaacc
     2821 tgtctcgctt ttcccctccg ctgcggccac tccctgaacc tcagaagagg aggactggag
     2881 agaggacgaa gacggtagta cagcctttcc ttcagtggtg ctttcagcag ccagcagtcc
     2941 caagtgcagc ctccttgtcc aggggccctg ttctgggggc tggggggtgt gagtaggcgg
     3001 cagggacgga gtgggtcagt cgtttcctcc cctgcctccc aggggccagg atcacagttc
     3061 caagctgatc atcatgcctg aacttcaggg ccactggctc ctcttcctgg ttcctggatc
     3121 cttcccctgg gttgggtggt gggagggacc aaggctagct ggtctcccaa gctgctccaa
     3181 atgtggccat agaggggacc tggcacctgg aagacagcag cgccaccctg cccccgcgca
     3241 caccccagtg ttccatgctg ggcctctaga ctagtcgagc tgcccgaaca acttcagggc
     3301 cgcatctcat ttgccagcag gggggatgca aaaaatccca cctatggtgc tgatgttcaa
     3361 tgaatgcatt gggtgcccac tagcccagtg acccagggaa ggctaggaaa gcactccgag
     3421 acaaagtagc acttttcaga aggagaataa gccctgggct tggagtcctt gaggaatctg
     3481 agcaagattc ctctggttaa tggtgcacaa tatctctaag ctagaagctg aagctggttt
     3541 ggggaccaaa tgtgtgacaa gctgtgggat tgtgtgggga agaagaaaag atgaaggaat
     3601 gcatcgtaac ttaccttgtt ctcatttttc cctcctacag aaaaagagga gggcagccac
     3661 cgcccggcgg cagcacctga agagtgctat gctccagcaa tcttgtaggt tccaggctgc
     3721 tgaggacctg caccagccat gcaactttct attttgtaac aatttctggt tactgttgct
     3781 gcaaagctcc atgtgacaca gtgtatgtaa agtgtacata aattaattta ttttacctcg
     3841 ttttgtttgt ttttaaaacc aatgccctgt ggaaggaaac ataaaacttc aagaagcatt
     3901 aaatcatcag tcattctgtc acacccctaa tgcagttgtt tctgtcatca tttccctggg
     3961 ctcttccatc tctcgctgac ctgggactgg gtgctggggc tgggagcagg ggttggggct
     4021 ctccagggag agatggcatg gggagagtga tgggatactg ctgggggggg gggactcacc
     4081 ctgctgtggg ctgcaggaag cccattggtg cagagagcag cctgggatgc ccatgacacg
     4141 ggcacccact gcaccgtgtt tctcccatgc ccagtaggga aagggttacg agcgccgttc
     4201 attctcagct tgtgaaggat tttgttgggc tcagcctgcc agagcagtag ccaggcatgc
     4261 ctgtgcagct ccgagctgtg atggacagag gcaaggctgc agctagagcg gccgccaccg
     4321 cggtggagct ccaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta
     4381 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4441 cccttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4501 cgtagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4681 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4861 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4921 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca
     4981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5461 agagaattat gcagtgctgc cataagcatg agtgataaca ctgcggccaa cttacttctg
     5521 acaacgatcg gaggaccgaa ggagctaacc gctttttttc acaacatggg ggatcatgta
     5581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5881 gttatctaca cgacgggcag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     6301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     6541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6601 acaggagagc gcacgaggga gcttccaggg gggaacgcct ggtatcttta tagtcctgtc
     6661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggccgagc
     6721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     6961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7021 gtgagttacc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg
     7081 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7141 gccaagctcg gaattaaccc tcactaaagg gaacaaaagc tg
//
