LOCUS       sequence-151617-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Zuger S, Iwai H
  TITLE     Intein-based biosynthetic incorporation of unlabeled protein tags 
            into isotopically labeled proteins for NMR studies.
  JOURNAL   Nat Biotechnol. 2005 Jun . 23(6):736-40.
  PUBMED    15908942
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Jun . 23(6):736-40."
COMMENT     Sequence Label: pJJDuet30
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    129..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             209..226
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             236..253
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc
      121 actatagggg aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt
      181 aataaggaga tataccatgg gcagcagcca tcatcatcat catcacagca gcggcctggt
      241 gccgcgcggc agccatatgc agtacaaact tatcctgaac ggtaaaaccc tgaaaggtga
      301 aaccaccacc gaagctgttg acgctgctac cgcggaaaaa gttttcaaac agtacgctaa
      361 cgacaacggt gttgacggtg aatggaccta cgacgacgct accaaaacct acaccgtaac
      421 ggaaggatcc tgcctcagtt ttggtaccga aattttaacc gttgagtacg gcccattgcc
      481 cattggcaaa attgtgagtg aagaaattaa ttgttctgtg tacagtgttg atccagaagg
      541 gagagtttac acccaggcga tcgcccaatg gcatgaccgg ggagagcagg aagtattgga
      601 atatgaattg gaagatggtt cagtaatccg agctacctct gaccaccgct ttttaaccac
      661 cgattatcaa ctgttggcga tcgaagaaat ttttgctagg caactggact tgttgacttt
      721 agaaaatatt aagcaaactg aagaagctct tgacaaccat cgtcttccct ttccattact
      781 tgacgcaggt acaattaaat aaataaagct tgcggccgca taatgcttaa gtcgaacaga
      841 aagtaatcgt attgtacacg gccgcataat cgaaattaat acgactcact atagg
//
