LOCUS       sequence-151618-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Zuger S, Iwai H
  TITLE     Intein-based biosynthetic incorporation of unlabeled protein tags 
            into isotopically labeled proteins for NMR studies.
  JOURNAL   Nat Biotechnol. 2005 Jun . 23(6):736-40.
  PUBMED    15908942
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2005 Jun . 23(6):736-40."
COMMENT     Sequence Label: pJJDuet30
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(5..49)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    158..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta ccagactcga
       61 gggtaccgac gtcagcgatc gcgtggccgg ccgatatcca attgagatct gccatatgta
      121 tatctccttc ttatacttaa ctaatatact aagatgggga attgttatcc gctcacaatt
      181 cccctatagt gagtcgtatt aatttcgatt atgcggccgt gtacaatacg attactttct
      241 gttcgactta agcattatgc ggccgcaagc tttatttatt taattgtacc tgcgtcaagt
      301 aatggaaagg gaagacgatg gttgtcaaga gcttcttcag tttgcttaat attttctaaa
      361 gtcaacaagt ccagttgcct agcaaaaatt tcttcgatcg ccaacagttg ataatcggtg
      421 gttaaaaagc ggtggtcaga ggtagctcgg attactgaac catcttccaa ttcatattcc
      481 aatacttcct gctctccccg gtcatgccat tgggcgatcg cctgggtgta aactctccct
      541 tctggatcaa cactgtacac agaacaatta atttcttcac tcacaatttt gccaatgggc
      601 aatgggccgt actcaacggt taaaatttcg gtaccaaaac tgaggcagga tccttccgtt
      661 acggtgtagg ttttggtagc gtcgtcgtag gtccattcac cgtcaacacc gttgtcgtta
      721 gcgtactgtt tgaaaacttt ttccgcggta gcagcgtcaa cagcttcggt ggtggtttca
      781 cctttcaggg ttttaccgtt caggataagt tt
//
