LOCUS       sequence-151663-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 gRNA variant to recruit ms2 proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pSB700 Cerulean-Zhang2.0
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     137..156
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        253..271
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        323..341
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     enhancer        445..748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cttcttcgat caggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat
       61 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
      121 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      181 ggctttatat atcttgtgga aaggacgaaa caccggagac gattaatgcg tctcggtttt
      241 agagctaggc caacatgagg atcacccatg tctgcagggc ctagcaagtt aaaataaggc
      301 tagtccgtta tcaacttggc caacatgagg atcacccatg tctgcagggc caagtggcac
      361 cgagtcggtg ctttttttga attctcgacc tcgagacaaa tggcagtatt cgtcattagt
      421 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      481 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      541 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      601 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      661 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      721 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
      781 ccccatctcc ccc
//
